F31FP302

Anti-Rat Foxp3, PE (Clone: FJK-16s) 检测试剂

¥1,380.00 – ¥2,880.00

说明书

分子靶点：FOXP3, IPEX
种属：人 (Human)
标记：PE
亚型：Rat IgG2a, κ
克隆号：FJK-16s

在售SKU, 70-F31FP302-25, 70-F31FP302-100, F31FP302

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
FOXP3 分子靶点信息概述
FOXP3 分子靶点综述
- 大鼠 Rat Foxp3 分子靶点信息
- 大鼠 Rat Foxp3 靶点分子功能（预测）

商品名	Anti-Rat Foxp3, PE (Clone: FJK-16s) 检测试剂
靶点	FOXP3, IPEX
标记	PE
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Rat IgG2a, κ
克隆号	FJK-16s
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含0.2% BSA和 0.2% ProClin950的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 以大鼠血液CD4阳性细胞设门进行分析的结果示例（需要配合使用Transcription Factor Fixation/Permeabilization Buffer），左图为Anti-Rat CD4, APC和Rat IgG2a, PE染色结果，右图为Anti-Rat CD4, APC和Anti-Rat Foxp3, PE染色结果。

分子信息

概述大鼠 FOXP3, IPEX 靶点信息

FOXP3 分子靶点信息概述

分子名：FOXP3, forkhead box P3
基因家族：Forkhead boxes
别名：JM2; XPID; AIID; PIDX; DIETER; SCURFIN
曾用名：IPEX
全称：immune dysregulation, polyendocrinopathy, enteropathy, X-linked

FOXP3 分子靶点综述

FoxP3蛋白是转录因子叉头家族的成员之一，表达于调节性T细胞(Regulatory T Cells, Treg)，其发育和功能受到叉头蛋白的影响。T细胞中FoxP3的异常表达会抑制其活性和细胞因子分泌。

大鼠 Rat Foxp3 分子靶点信息

分子名：Foxp3, forkhead box P3
别称：
- forkhead box protein P3
- LOC317382
- RGD1562112
- similar to scurfin
基因序列：NCBI_Gene: 317382
蛋白序列：

大鼠 Rat Foxp3 靶点分子功能（预测）

Predicted to enable several functions, including DNA-binding transcription repressor activity, RNA polymerase II-specific; NFAT protein binding activity; and enzyme binding activity. Involved in several processes, including establishment of endothelial blood-brain barrier; negative regulation of defense response to virus; and response to rapamycin. Predicted to be located in cytoplasm and nucleoplasm. Predicted to be active in nucleus. Used to study intrahepatic cholangiocarcinoma. Biomarker of Korean hemorrhagic fever; asthma; bronchiolitis obliterans; hypertension; and hypothyroidism. Human ortholog(s) of this gene implicated in Human papillomavirus infectious disease; colorectal cancer; immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome; pulmonary tuberculosis; and type 1 diabetes mellitus. Orthologous to human FOXP3 (forkhead box P3).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

1.收集细胞，调整细胞浓度到10⁶ -10⁷个/ml。
2. 在流式管中加入 50 μl 细胞，加入表染抗体。涡旋震荡混匀，室温避光孵育 15 分钟。
3. 每管加入 2 ml 1× FCM Lysing Solution 工作液，涡旋震荡混匀，室温避光孵育 15 分钟。室温下 300 - 400 × g 离心 5 分钟，弃去上清。
4. 每管加入 2 ml 1× Flow Cytometry Staining Buffer，涡旋震荡混匀。室温下 300 - 400 × g 离心 5分钟，弃去上清。
5. 每管加入 1 ml 1× Fixation/Permeabilization 工作液，涡旋震荡混匀。
6. 室温避光孵育 30 - 60 分钟。
7. 无需洗涤，每管加入 2 ml 1× Permeabilization Buffer。室温下300 - 400 × g 离心 5 分钟，弃去上清。
8. 用100 μl 1× Permeabilization Buffer重悬沉淀。通常洗涤后残留的液体量就已足够。
9.加入5 μl Anti-Rat Foxp3, PE或5 μl Rat IgG2a Isotype Control, PE。震荡混匀，室温避光孵育至少30分钟。
10.每管加入2 ml 1× Permeabilization Buffer，室温下300 - 400 × g离心5分钟，弃去上清。
11.每管加入 500 μl 1× Flow Cytometry Staining Buffer 重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。实验中，可对抗体进行优化滴定以获得最佳效果。