F21FP302

Anti-Mouse FoxP3, PE (Clone:3G3)流式抗体 (新品)

¥720.00 – ¥1,584.00

说明书

分子靶点：FOXP3, IPEX
种属：小鼠 (Mouse)
标记：PE
亚型：Mouse IgG1, κ
克隆号：3G3

在售SKU, 70-AM0F04-20, 70-AM0F04-50, 70-AM0F04-100, AM0F04, 70-F21FP302-25, 70-F21FP302-100

¥<\/span>1,584.00<\/bdi><\/span><\/span>","sku":"70-F21FP302-100","variation_description":"","variation_id":15729,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"25T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":720,"display_regular_price":720,"image":{"title":"1656571413-fc-antibody.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":16568,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>720.00<\/bdi><\/span><\/span>","sku":"70-F21FP302-25","variation_description":"","variation_id":15730,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

分子信息
FOXP3 分子靶点信息概述
FOXP3 分子靶点综述
- 小鼠 Mouse Foxp3 分子靶点信息
- 小鼠 Mouse Foxp3 靶点分子功能（预测）

商品名	Anti-Mouse FoxP3, PE (Clone:3G3)流式抗体
靶点	FOXP3, IPEX
标记	PE
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	3G3
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

分子信息

概述小鼠 FOXP3, IPEX 靶点信息

FOXP3 分子靶点信息概述

分子名：FOXP3, forkhead box P3
基因家族：Forkhead boxes
别名：JM2; XPID; AIID; PIDX; DIETER; SCURFIN
曾用名：IPEX
全称：immune dysregulation, polyendocrinopathy, enteropathy, X-linked

FOXP3 分子靶点综述

FoxP3蛋白是转录因子叉头家族的成员之一，表达于调节性T细胞(Regulatory T Cells, Treg)，其发育和功能受到叉头蛋白的影响。T细胞中FoxP3的异常表达会抑制其活性和细胞因子分泌。

小鼠 Mouse Foxp3 分子靶点信息

分子名：Foxp3, forkhead box P3
别称：
- JM2
- scurfin
- scurfy
- sf
基因序列：NCBI_Gene: 20371
蛋白序列：

小鼠 Mouse Foxp3 靶点分子功能（预测）

Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; identical protein binding activity; and transcription corepressor activity. Involved in negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; and regulation of T cell anergy. Acts upstream of or within several processes, including positive regulation of T cell tolerance induction; regulation of T cell activation; and regulation of gene expression. Located in cytoplasm and nucleus. Is expressed in central nervous system; genitourinary system; hemolymphoid system; and retina. Used to study Wiskott-Aldrich syndrome and immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome. Human ortholog(s) of this gene implicated in Human papillomavirus infectious disease; colorectal cancer; immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome; pulmonary tuberculosis; and type 1 diabetes mellitus. Orthologous to human FOXP3 (forkhead box P3).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

取 100 μl 抗凝血或含有 1×10⁵ - 10⁸ 个单细胞的细胞悬液，根据实验方案和产品说明书，加入适量抗体进行表面染色，震荡混匀，于室温避光孵育适当时间。
将 Fixation/Permeabilization Concentrate (4×) (组分编号 ICC01)和 Fixation/Permeabilization Diluent(组分编号 ICD01)按 1：3 比例制备成 1×工作液。在流式管中染色，每个样本需要 1 ml。保存勿超过 1 天。用蒸馏水将 Permeabilization Buffer (10×) (组分编号 ICB01)稀释为 1×工作液。在流式管中染色，每个样本需要 8.5 ml。剩余溶液在 2 - 8℃最多保存 1 周。准备加入 1 ml
每管加入 1 ml Fixation/Permeabilization 工作液，涡旋震荡混匀。室温避光孵育 30 - 60 分钟。
无需洗涤，每管加入 2 ml 1× Permeabilization Buffer。室温下 300 - 400 × g 离心 5 分钟，弃去上清。
(可选)重复步骤 4。
用 100 μl 1× Permeabilization Buffer 重悬沉淀。通常洗涤后残留的液体量就已足够。
(可选)细胞悬液中加入 2 μl Anti-Mouse CD16/CD32, Purified (目录号 AM016)进行阻断，室温避光孵育 15 分钟。
无需洗涤，加入 5 μl Anti-Mouse Foxp3, PE 或 0.125 μg Mouse IgG1 Isotype Control, PE (目录号CMG104)。震荡混匀，室温避光孵育 15 分钟。
每管加入 2 ml 1× Permeabilization Buffer，室温下 300 - 400 × g 离心 5 分钟，弃去上清。
(可选)重复步骤 9。
每管加入 500 μl 1× Flow Cytometry Staining Buffer (目录号 S1001)重悬，上机检测。