F11062P01

Anti-Human CD62P, FITC (Clone:AK-4) (新品)

X

¥720.00¥1,584.00

  • 分子靶点:SELP, CD62P, GRMP
  • 种属:人 (Human)
  • 标记:FITC
  • 亚型:Mouse IgG1, κ
  • 克隆号:AK-4

在售SKU, 70-AH062P01-20, 70-AH062P01-50, 70-AH062P01-100, AH062P01, 70-F11062P01-25, 70-F11062P01-100

¥<\/span>1,584.00<\/bdi><\/span><\/span>","sku":"70-F11062P01-100","variation_description":"","variation_id":15380,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"25T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":720,"display_regular_price":720,"image":{"title":"1656571413-fc-antibody.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":16568,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>720.00<\/bdi><\/span><\/span>","sku":"70-F11062P01-25","variation_description":"","variation_id":15381,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

Anti-Human CD62P, FITC (Clone:AK-4)

靶点

SELP, CD62P, GRMP

标记

FITC

仪器

适用488nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

AK-4

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD62P, FITC (Clone:AK-4) - 结果示例图片
结果示例

图 - 人外周血染色 Anti-Human CD62P, FITC (F11062P01)(实线) 和 Mouse IgG1 κ Isotype Control FITC(CMG101) (虚线)的结果。以血小板设门进行分析。

分子信息

概述人 SELP, CD62P, GRMP 靶点信息

SELP 分子靶点信息概述

  • 分子名:SELP, selectin P
  • 基因家族:CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
  • 别名:CD62; PSEL; PADGEM; GMP140; CD62P
  • 曾用名:GRMP
  • 全称:antigen CD62; granule membrane protein 140kDa; selectin P (granule membrane protein 140kD, antigen CD62); selectin P (granule membrane protein 140kDa, antigen CD62)

SELP 分子靶点综述

CD62P 是一种 140kDa 跨膜糖蛋白,也称为 P-Selectin。P-Selectin被储存在血小板的 α 颗粒和内皮细胞的 Weibel-Palade 小体中,并在激活后迅速转运到质膜。在炎症反应中,P-Selectin 被认为介导中性粒细胞和单核细胞与内皮的初始粘附相互作用;在止血过程中,活化的血小板与中性粒细胞和单核细胞相互作用。

人 Human SELP 分子靶点信息

  • 分子名:SELP, selectin P
  • 别称:
    • CD62
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP-140
    • GMP140
    • granule membrane protein 140
    • granule membrane protein 140kDa
    • granulocyte membrane protein
    • GRMP
    • LECAM3
    • leukocyte-endothelial cell adhesion molecule 3
    • P-selectin
    • PADGEM
    • platelet activation dependent granule-external membrane protein
    • platelet alpha-granule membrane protein
    • PSEL
    • selectin P (granule membrane protein 140kDa, antigen CD62)
  • 基因序列:NCBI_Gene: 6403
  • 蛋白序列:UniProtKB: P16109

人 Human SELP靶点分子功能(预测)

Enables several functions, including fucose binding activity; heparin binding activity; and lipopolysaccharide binding activity. Involved in several processes, including calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; calcium-mediated signaling using intracellular calcium source; and leukocyte tethering or rolling. Located in external side of plasma membrane; extracellular space; and platelet alpha granule membrane. Is integral component of plasma membrane. Implicated in atopic dermatitis and myocardial infarction. Biomarker of several diseases, including Kawasaki disease; autoimmune disease (multiple); coronary artery disease (multiple); obesity; and type 2 diabetes mellitus.

操作步骤


下载本产品说明书

使用方法

全血样本:在流式管中加入 100μl 枸橼酸钠(ACD)抗凝血,与 2 ml 洗液(PBS, pH7.2 - 7.4,含 0.35% BSA和 13 mM 枸橼酸钠)混匀,300 × g 离心 5 分钟,弃上清,洗涤 2 次。用 100 μl 洗液重悬沉淀(通常洗涤后残留的液体量就已足够)。加入 10 μl 凝血酶溶液(100U/ml),于 37℃孵育 5 分钟。孵育结束后,无需洗涤,直接加入 5 μl Anti-Human CD62P, FITC 和其他抗体,震荡混匀,室温避光孵育 15 分钟。加入 2 ml 1%多聚甲醛,震荡混匀,避光孵育 15 分钟。300 × g 离心 5分钟,弃上清。每管加入 2 ml 洗液,震荡混匀,300 × g 离心 5 - 10 分钟,弃上清,加入 500μl洗液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

查看更多FCM流式检测相关问题

引用文献


评价

目前还没有评价

成为第一个“Anti-Human CD62P, FITC (Clone:AK-4) (新品)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注