F1127902

Anti-Human CD279 (PD-1), PE (Clone:EH12.2H7) 流式抗体检测试剂

¥792.00 – ¥1,760.00

说明书

分子靶点：PDCD1, PD1, CD279
种属：人 (Human)
标记：PE
亚型：Mouse IgG1, κ
克隆号：EH12.2H7

在售SKU, F1127902, 70-F1127902-25, 70-F1127902-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
PDCD1 分子靶点信息概述
PDCD1 分子靶点综述
- 人 Human PDCD1 分子靶点信息
- 人 Human PDCD1靶点分子功能（预测）

商品名	Anti-Human CD279 (PD-1), PE (Clone:EH12.2H7)检测试剂
靶点	PDCD1, PD1, CD279
标记	PE
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	EH12.2H7
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人 PBMC 经 Anti-Human CD3 Monoclonal Antibody, Functional Grade (F1100300) 和Anti-Human CD28, Monoclonal Antibody, Functional Grade (F1102800)刺激 3 天后，染色Anti-Human CD4, APC (F11004B03) 和 Mouse IgG1 Isotype Control, PE (CMG104) ( 左) 和Anti-Human CD4, APC (F11004B03) 和Anti-Human CD279, PE(F1127902) (右)的结果。已排除粘连细胞。

分子信息

概述人 PDCD1, PD1, CD279 靶点信息

PDCD1 分子靶点信息概述

分子名：PDCD1, programmed cell death 1
基因家族：CD molecules; V-set domain containing
别名：CD279; PD1; hSLE1; PD-1
曾用名：SLEB2
全称：systemic lupus erythematosus susceptibility 2

PDCD1 分子靶点综述

CD279 也称为程序性细胞死亡蛋白 1(PD-1)，是免疫球蛋白超家族的一员，分子量 55 kD。其胞浆区含有免疫受体酪氨酸抑制基序(ITIM)，在外周耐受和自身免疫疾病中具有重要作用。CD279 主要表达于活化的 T 细胞、B 细胞和髓系细胞中。PD-L1(B7-H1)和 PD-L2(B7-DC)是 CD279(PD-1)的配体，属于 B7 基因家族。这两个 PD-1 配体具有重叠的功能，在某些正常组织中组成性表达，在活化的抗原提呈细胞中表达上调。与 CD279 配体的作用可抑制 T 细胞增殖和细胞因子分泌。

人 Human PDCD1 分子靶点信息

分子名：PDCD1, programmed cell death 1
别称：
- CD279
- hPD-1
- hPD-l
- hSLE1
- PD-1
- PD1
- programmed cell death protein 1
- SLEB2
- systemic lupus erythematosus susceptibility 2
基因序列：NCBI_Gene: 5133
蛋白序列：UniProtKB: Q15116

人 Human PDCD1靶点分子功能（预测）

Involved in positive regulation of T cell apoptotic process. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Biomarker of several diseases, including Cryptococcal meningitis; anogenital venereal wart; cervix uteri carcinoma in situ; liver disease (multiple); and tuberculosis (multiple).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD279, PE 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃ 避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD279, PE 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃ 避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。