F1127902

Anti-Human CD279 (PD-1), PE (Clone:EH12.2H7) 流式抗体 检测试剂

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¥792.00¥1,760.00

  • 分子靶点:PDCD1, PD1, CD279
  • 种属:人 (Human)
  • 标记:PE
  • 亚型:Mouse IgG1, κ
  • 克隆号:EH12.2H7

在售SKU, F1127902, 70-F1127902-25, 70-F1127902-100

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描述

商品名

Anti-Human CD279 (PD-1), PE (Clone:EH12.2H7)检测试剂

靶点

PDCD1, PD1, CD279

标记

PE

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

EH12.2H7

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD279 (PD-1), PE (Clone:EH12.2H7) 流式抗体 检测试剂 - 结果示例图片Anti-Human CD279 (PD-1), PE (Clone:EH12.2H7) 流式抗体 检测试剂 - 结果示例图片
结果示例

图 - 人 PBMC 经 Anti-Human CD3 Monoclonal Antibody, Functional Grade (F1100300) 和Anti-Human CD28, Monoclonal Antibody, Functional Grade (F1102800)刺激 3 天后,染色Anti-Human CD4, APC (F11004B03) 和 Mouse IgG1 Isotype Control, PE (CMG104) ( 左) 和Anti-Human CD4, APC (F11004B03) 和Anti-Human CD279, PE(F1127902) (右)的结果。已排除粘连细胞。

分子信息

概述人 PDCD1, PD1, CD279 靶点信息

PDCD1 分子靶点信息概述

  • 分子名:PDCD1, programmed cell death 1
  • 基因家族:CD molecules; V-set domain containing
  • 别名:CD279; PD1; hSLE1; PD-1
  • 曾用名:SLEB2
  • 全称:systemic lupus erythematosus susceptibility 2

PDCD1 分子靶点综述

CD279 也称为程序性细胞死亡蛋白 1(PD-1),是免疫球蛋白超家族的一员,分子量 55 kD。其胞浆区含有免疫受体酪氨酸抑制基序(ITIM),在外周耐受和自身免疫疾病中具有重要作用。CD279 主要表达于活化的 T 细胞、B 细胞和髓系细胞中。PD-L1(B7-H1)和 PD-L2(B7-DC)是 CD279(PD-1)的配体,属于 B7 基因家族。这两个 PD-1 配体具有重叠的功能,在某些正常组织中组成性表达,在活化的抗原提呈细胞中表达上调。与 CD279 配体的作用可抑制 T 细胞增殖和细胞因子分泌。

人 Human PDCD1 分子靶点信息

  • 分子名:PDCD1, programmed cell death 1
  • 别称:
    • CD279
    • hPD-1
    • hPD-l
    • hSLE1
    • PD-1
    • PD1
    • programmed cell death protein 1
    • SLEB2
    • systemic lupus erythematosus susceptibility 2
  • 基因序列:NCBI_Gene: 5133
  • 蛋白序列:UniProtKB: Q15116

人 Human PDCD1靶点分子功能(预测)

Involved in positive regulation of T cell apoptotic process. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Implicated in autoimmune disease (multiple); hepatitis B; hepatitis C; hepatocellular carcinoma; and lupus nephritis. Biomarker of several diseases, including Cryptococcal meningitis; anogenital venereal wart; cervix uteri carcinoma in situ; liver disease (multiple); and tuberculosis (multiple).

操作步骤


下载本产品说明书

使用方法

全血样本:取 100 μl 抗凝血,加入 5 μl Anti-Human CD279, PE 和其他抗体,震荡混匀,于室温避光孵育 15 分钟或 2 - 8℃ 避光孵育 30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号 S1001)重悬,加入 5 μl Anti-Human CD279, PE 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃ 避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

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