F1107101

Anti-Human CD71, FITC(Clone:OKT9) (新品)

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¥872.00¥1,936.00

  • 分子靶点:TFRC, CD71, TFR1
  • 种属:人 (Human)
  • 标记:FITC
  • 亚型:Mouse IgG1, κ
  • 克隆号:OKT9

在售SKU, 70-AH07101-20, 70-AH07101-50, 70-AH07101-100, AH07101, 70-F1107101-25, 70-F1107101-100

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描述

商品名

Anti-Human CD71, FITC(Clone:OKT9)

靶点

TFRC, CD71, TFR1

标记

FITC

仪器

适用488nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

OKT9

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD71, FITC(Clone:OKT9) - 结果示例图片Anti-Human CD71, FITC(Clone:OKT9) - 结果示例图片
结果示例

图 - 正常人外周血经淋巴细胞分离液(LSM01) 分离,以 Anti-Human CD3, Functional Grade (F1100300)Anti-Human CD28, Functional Grade (F1102800) 刺激培养 48 小时后,染色 Anti-Human CD25, APC (Clone:BC96) (F1102503) 和 Anti-Human CD71, FITC (F1107101)的结果。以 FSC/SSC 中的增殖的淋巴细胞设门进行分析。

分子信息

概述人 TFRC, CD71, TFR1 靶点信息

TFRC 分子靶点信息概述

  • 分子名:TFRC, transferrin receptor
  • 基因家族:CD molecules; M28 metallopeptidases
  • 别名:CD71; TFR1; p90
  • 全称:transferrin receptor (p90, CD71)

TFRC 分子靶点综述

转铁蛋白受体 CD71 是一种 1180 kDa 的 II 型跨膜蛋白,在细胞表面以同源二聚体的形式存在,对细胞生长至关重要。CD71 表达于未成熟的增殖中的细胞,在静息的成熟淋巴细胞中表达水平较低。抗原或丝裂原刺激的 T 细胞和 B 细胞可上调 CD71 的表达。

人 Human TFRC 分子靶点信息

  • 分子名:TFRC, transferrin receptor
  • 别称:
    • CD71
    • IMD46
    • p90
    • T9
    • TFR
    • TFR1
    • TR
    • transferrin receptor (p90, CD71)
    • transferrin receptor protein 1
    • TRFR
  • 基因序列:NCBI_Gene: 7037
  • 蛋白序列:UniProtKB: P02786

人 Human TFRC靶点分子功能(预测)

Enables several functions, including double-stranded RNA binding activity; protein homodimerization activity; and transferrin receptor activity. Involved in several processes, including negative regulation of mitochondrial fusion; positive regulation of NF-kappaB transcription factor activity; and positive regulation of lymphocyte activation. Acts upstream of or within cellular response to xenobiotic stimulus. Located in several cellular components, including basolateral plasma membrane; clathrin-coated pit; and endosome. Is integral component of plasma membrane. Part of HFE-transferrin receptor complex. Implicated in breast carcinoma; immunodeficiency 46; multiple myeloma; and ovarian cancer. Biomarker of breast cancer; carcinoma (multiple); iron deficiency anemia; and prostate cancer.

操作步骤


下载本产品说明书

使用方法

全血样本:取 100 μl 适当处理的血液样本,加入 5 μl Anti-Human CD71, FITC 和其它抗体,震荡混匀,于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。300 × g 离心 5 - 10 分钟,弃上清,加入500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号 S1001)重悬,加入 5 μl Anti-Human CD71, FITC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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