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2024年6月18日，南方医科大学石敏、廖旺军研究团队在Cell子刊Cell Metabolism（IF=27.7）上发表了标题为“Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer”的研究文章，通过转录组分析和动态血浆样本分析，研究团队识别出了肿瘤微环境中的一种代谢“对决”机制，揭示了巨噬细胞衍生的细胞外囊泡（EVs）具有类似外泌体的结构，并携带烟酰胺磷酸核糖转移酶（NAMPT）作为货物来发挥抗肿瘤效果。
此论文使用了联科生物Anti-Mouse F4/80, PE-Cy7 (Clone:BM8.1)（货号：F21480A05）、Anti-Mouse CD8α, PerCP-Cy5.5 (Clone:2.43)（货号：F21008A04）、Anti-Mouse IFN-γ, PE-Cy7 (Clone:XMG1.2) （货号：F21IFNG05）和Anti-Human CD8, PerCP-Cy5.5 (Clone:HIT8a) （货号：F11008C04）4种流式抗体。
(K) Mouse CAFs were treated with BMDM-derived EVs or Selisistat and then cocultured with CD8+ T cells for 24 h. Flow cytometry detected the cytotoxicitymarkers (IFN-g and GZMB) on T cells
此外，巨噬细胞衍生的囊泡被认为是理想的药物载体，具有高趋向性和肿瘤归巢能力。他们发现，巨噬细胞衍生的EVs具有良好的成纤维细胞（CAF）靶向潜力，因此在动物实验中将其用于联合治疗方案。体外改造的自体巨噬细胞衍生EVs成功抑制了肿瘤生长，并将代谢流转向有利的抗肿瘤状态。他们的发现为基于代谢的联合治疗方法的开发提供了基础。总体而言，这项研究强调了烟酰胺代谢作为免疫检查点抑制（ICB）生物标志物的重要性，并提供了对胃癌肿瘤微环境中内在代谢互作的见解。
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参考文献
Jiang Y, Wang Y, Chen G, et al. Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer[J]. Cell Metabolism, 2024.
原文链接
https://www.cell.com/cell-metabolism/fulltext/S1550-4131(24)00189-X
