F2100304

Anti-Mouse CD3ε, PerCP-Cy5.5 (Clone:145-2C11) 流式抗体 检测试剂

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¥600.00¥1,320.00

  • 分子靶点:CD3E, CD3 epsilon, CD3ε
  • 种属:小鼠 (Mouse)
  • 标记:PerCP-Cy5.5
  • 亚型:Armenian Hamster IgG
  • 克隆号:145-2C11

在售SKU, F2100304, 70-F2100304-25, 70-F2100304-100

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描述

商品名

Anti-Mouse CD3ε, PerCP-Cy5.5 (Clone:145-2C11) 检测试剂

靶点

CD3E, CD3 epsilon, CD3ε

标记

PerCP-Cy5.5

仪器

适用488nm激光器的流式细胞仪

亚型

Armenian Hamster IgG

克隆号

145-2C11

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Mouse CD3ε, PerCP-Cy5.5 (Clone:145-2C11) 流式抗体 - 结果示例图片
结果示例

图 - 图. ICR 小鼠脾细胞染色 Anti-Mouse CD3ε, PerCP-Cy5.5 (F2100304) 和 Anti-Mouse CD19, FITC (F2101901) 的结果。已排除黏连细胞。

分子信息

概述小鼠 CD3E, CD3 epsilon, CD3ε 靶点信息

CD3E 分子靶点信息概述

  • 分子名:CD3E, CD3e molecule
  • 基因家族:CD molecules
  • 全称:CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex)

CD3E 分子靶点综述

CD3也称为T3,是免疫球蛋白超家族的一员,在抗原识别、信号传导和T细胞活化中具有重要作用。CD3ε的分子量为20 kD,是CD3分子的亚基,后者由2个CD3ε、1个CD3γ、1个CD3δ和1个CD3ζ (CD247)构成,并与α/β或γ/δT细胞受体(TCR)形成异源二聚体。CD3表达于所有成熟T细胞、NKT细胞和部分胸腺细胞。

小鼠 Mouse Cd3e 分子靶点信息

小鼠 Mouse Cd3e 靶点分子功能(预测)

Predicted to enable several functions, including SH3 domain binding activity; identical protein binding activity; and protein heterodimerization activity. Involved in nervous system development and positive regulation of cell adhesion. Acts upstream of or within several processes, including positive regulation of T cell activation; positive regulation of cytokine production; and regulation of signal transduction. Located in several cellular components, including dendritic spine; external side of plasma membrane; and immunological synapse. Part of alpha-beta T cell receptor complex. Is expressed in colon and hemolymphoid system. Human ortholog(s) of this gene implicated in immunodeficiency 18. Orthologous to human CD3E (CD3e molecule).

操作步骤


下载本产品说明书

使用方法

全血样本:取 50 - 100 μl 抗凝血,加入 5 μl Anti-Mouse CD3ε, PerCP-Cy5.5 和其他抗体,震荡混匀,于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液(目录号S1001)重悬,加入5μl Anti-Mouse CD3ε, PerCP-Cy5.5 和其他抗体,使反应终体积为100 μl。震荡混匀,于室温避光孵育 15 分钟或2 - 8℃避光孵育30 分钟。每管加入1 - 2 ml 流式染色缓冲液,300×g离心5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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