F2100304

Anti-Mouse CD3ε, PerCP-Cy5.5 (Clone:145-2C11) 流式抗体检测试剂

¥600.00 – ¥1,320.00

说明书

分子靶点：CD3E, CD3 epsilon, CD3ε
种属：小鼠 (Mouse)
标记：PerCP-Cy5.5
亚型：Armenian Hamster IgG
克隆号：145-2C11

在售SKU, F2100304, 70-F2100304-25, 70-F2100304-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD3E 分子靶点信息概述
CD3E 分子靶点综述
- 小鼠 Mouse Cd3e 分子靶点信息
- 小鼠 Mouse Cd3e 靶点分子功能（预测）

商品名	Anti-Mouse CD3ε, PerCP-Cy5.5 (Clone:145-2C11) 检测试剂
靶点	CD3E, CD3 epsilon, CD3ε
标记	PerCP-Cy5.5
仪器	适用488nm激光器的流式细胞仪
亚型	Armenian Hamster IgG
克隆号	145-2C11
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 图. ICR 小鼠脾细胞染色 Anti-Mouse CD3ε, PerCP-Cy5.5 (F2100304) 和 Anti-Mouse CD19, FITC (F2101901) 的结果。已排除黏连细胞。

分子信息

概述小鼠 CD3E, CD3 epsilon, CD3ε 靶点信息

CD3E 分子靶点信息概述

分子名：CD3E, CD3e molecule
基因家族：CD molecules
全称：CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex)

CD3E 分子靶点综述

CD3也称为T3，是免疫球蛋白超家族的一员，在抗原识别、信号传导和T细胞活化中具有重要作用。CD3ε的分子量为20 kD，是CD3分子的亚基，后者由2个CD3ε、1个CD3γ、1个CD3δ和1个CD3ζ (CD247)构成，并与α/β或γ/δT细胞受体(TCR)形成异源二聚体。CD3表达于所有成熟T细胞、NKT细胞和部分胸腺细胞。

小鼠 Mouse Cd3e 分子靶点信息

分子名：Cd3e, CD3 antigen, epsilon polypeptide
别称：
- AI504783
- CD3
- CD3epsilon
- expressed sequence AI504783
- T3-epsilon chain
- T3e
基因序列：NCBI_Gene: 12501
蛋白序列：

小鼠 Mouse Cd3e 靶点分子功能（预测）

Predicted to enable several functions, including SH3 domain binding activity; identical protein binding activity; and protein heterodimerization activity. Involved in nervous system development and positive regulation of cell adhesion. Acts upstream of or within several processes, including positive regulation of T cell activation; positive regulation of cytokine production; and regulation of signal transduction. Located in several cellular components, including dendritic spine; external side of plasma membrane; and immunological synapse. Part of alpha-beta T cell receptor complex. Is expressed in colon and hemolymphoid system. Human ortholog(s) of this gene implicated in immunodeficiency 18. Orthologous to human CD3E (CD3e molecule).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 50 - 100 μl 抗凝血，加入 5 μl Anti-Mouse CD3ε, PerCP-Cy5.5 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液(目录号S1001)重悬，加入5μl Anti-Mouse CD3ε, PerCP-Cy5.5 和其他抗体，使反应终体积为100 μl。震荡混匀，于室温避光孵育 15 分钟或2 - 8℃避光孵育30 分钟。每管加入1 - 2 ml 流式染色缓冲液，300×g离心5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。