F11008A04

Anti-Human CD8α, PerCP-Cy5.5 (Clone: SK1) 流式抗体 (新品)

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¥1,404.00¥3,120.00

  • 分子靶点:CD8A, CD8
  • 种属:人 (Human)
  • 标记:PerCP-Cy5.5
  • 亚型:Mouse IgG2, κ
  • 克隆号:SK1

在售SKU:70-F11008A04-25, 70-F11008A04-100

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描述

商品名

Anti-Human CD8α, PerCP-Cy5.5 (Clone: SK1) 流式抗体

靶点

CD8A, CD8

标记

PerCP-Cy5.5

仪器

适用488nm激光器的流式细胞仪

亚型

Mouse IgG2, κ

克隆号

SK1

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD8α, PerCP-Cy5.5 (Clone: SK1) 流式抗体 - 结果示例图片
结果示例

图 - 图. 人外周血染色 Anti-Human CD8α, PerCP-Cy5.5 (F11008A04)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD8A, CD8 靶点信息

CD8A 分子靶点信息概述

  • 分子名:CD8A, CD8a molecule
  • 基因家族:CD molecules; V-set domain containing
  • 曾用名:CD8
  • 全称:CD8 antigen, alpha polypeptide (p32); T-cell surface glycoprotein CD8 alpha chain

CD8A 分子靶点综述

CD8也称为T8或Leu2,是免疫球蛋白超家族的一员,表达于大部分胸腺细胞、外周血T细胞亚群和NK细胞(基本为CD8α/α同源二聚体)。CD8在抗原识别和T细胞活化中,作为MHC I类分子受限的T细胞受体,并在胸腺分化中具有重要作用。

人 Human CD8A 分子靶点信息

  • 分子名:CD8A, CD8a molecule
  • 别称:
    • CD8
    • cd8 antigen, alpha chain
    • CD8 antigen, alpha polypeptide (p32)
    • Cluster of Differentiation 8a
    • Leu2
    • Leu2 T-lymphocyte antigen
    • MAL
    • OKT8 T-cell antigen
    • p32
    • T cell co-receptor
    • T-cell antigen Leu2
    • T-cell surface glycoprotein CD8 alpha chain
    • T-lymphocyte differentiation antigen T8/Leu-2
    • T8 T-cell antigen
  • 基因序列:NCBI_Gene: 925
  • 蛋白序列:UniProtKB: P01732

人 Human CD8A靶点分子功能(预测)

Enables MHC class I protein complex binding activity. Predicted to be involved in T cell mediated immunity; cell surface receptor signaling pathway; and cytotoxic T cell differentiation. Predicted to act upstream of or within defense response to virus and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane and plasma membrane raft. Biomarker of COVID-19; erythema multiforme; and stomach cancer.

操作步骤


下载本产品说明书

使用方法

全血样本:取 100 μl 抗凝血,加入 5 μl Anti-Human CD8α, PerCP-Cy5.5 和其他抗体,震荡混匀,于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号 S1001)重悬,加入 5 μl Anti-Human CD8α, PerCP-Cy5.5 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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