F21FP303

Anti-Mouse FoxP3, APC (Clone:3G3)流式抗体 (新品)

X

¥720.00¥1,584.00

  • 分子靶点:FOXP3, IPEX
  • 种属:小鼠 (Mouse)
  • 标记:APC
  • 亚型:Mouse IgG1, κ
  • 克隆号:3G3

在售SKU, 70-AM0F05-20, 70-AM0F05-50, 70-AM0F05-100, AM0F05, 70-F21FP303-25, 70-F21FP303-100

¥<\/span>1,584.00<\/bdi><\/span><\/span>","sku":"70-F21FP303-100","variation_description":"","variation_id":15734,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"25T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":720,"display_regular_price":720,"image":{"title":"1656571413-fc-antibody.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":16568,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>720.00<\/bdi><\/span><\/span>","sku":"70-F21FP303-25","variation_description":"","variation_id":15735,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

Anti-Mouse FoxP3, APC (Clone:3G3)流式抗体

靶点

FOXP3, IPEX

标记

APC

仪器

适用633nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

3G3

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

分子信息

概述小鼠 FOXP3, IPEX 靶点信息

FOXP3 分子靶点信息概述

  • 分子名:FOXP3, forkhead box P3
  • 基因家族:Forkhead boxes
  • 别名:JM2; XPID; AIID; PIDX; DIETER; SCURFIN
  • 曾用名:IPEX
  • 全称:immune dysregulation, polyendocrinopathy, enteropathy, X-linked

FOXP3 分子靶点综述

FoxP3蛋白是转录因子叉头家族的成员之一,表达于调节性T细胞(Regulatory T Cells, Treg),其发育和功能受到叉头蛋白的影响。T细胞中FoxP3的异常表达会抑制其活性和细胞因子分泌。

小鼠 Mouse Foxp3 分子靶点信息

小鼠 Mouse Foxp3 靶点分子功能(预测)

Enables several functions, including DNA-binding transcription factor activity, RNA polymerase II-specific; identical protein binding activity; and transcription corepressor activity. Involved in negative regulation of interferon-gamma production; negative regulation of interleukin-17 production; and regulation of T cell anergy. Acts upstream of or within several processes, including positive regulation of T cell tolerance induction; regulation of T cell activation; and regulation of gene expression. Located in cytoplasm and nucleus. Is expressed in central nervous system; genitourinary system; hemolymphoid system; and retina. Used to study Wiskott-Aldrich syndrome and immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome. Human ortholog(s) of this gene implicated in Human papillomavirus infectious disease; colorectal cancer; immune dysregulation-polyendocrinopathy-enteropathy-X-linked syndrome; pulmonary tuberculosis; and type 1 diabetes mellitus. Orthologous to human FOXP3 (forkhead box P3).

操作步骤


下载本产品说明书

使用方法

  1. 取 100 μl 抗凝血或含有 1×105 - 108 个单细胞的细胞悬液,根据实验方案和产品说明书,加入适量抗体进行表面染色,震荡混匀,于室温避光孵育适当时间。
  2. 将 Fixation/Permeabilization Concentrate (4×) (Cat No. ICC01)和 Fixation/Permeabilization Diluent (CatNo. ICD01)按 1:3 比例制备成 1×工作液。在流式管中染色,每个样本需要 1 ml。保存勿超过 1 天。用蒸馏水将 Permeabilization Buffer (10×) (Cat No. ICB01)稀释为 1×工作液。在流式管中染色,每个样本需要 8.5 ml。剩余溶液在 2 - 8℃最多保存 1 周。准备加入 1 ml
  3. 每管加入 1 ml Fixation/Permeabilization 工作液,涡旋震荡混匀。室温避光孵育 30 - 60 分钟。
  4. 无需洗涤,每管加入 2 ml 1× Permeabilization Buffer。室温下 300 - 400 × g 离心 5 分钟,弃去上清。
  5. (可选)重复步骤 4。
  6. 用 100 μl 1× Permeabilization Buffer 重悬沉淀。通常洗涤后残留的液体量就已足够。
  7. (可选)细胞悬液中加入 2 μl Anti-Mouse CD16/CD32, Purified (Cat No. AM016)进行阻断,室温避光孵育 15 分钟。
  8. 无需洗涤,加入 5 μl Anti-Mouse Foxp3, APC 或 0.125 μg Mouse IgG1 Isotype Control, APC (Cat No.CMG105)。震荡混匀,室温避光孵育至少 30 分钟。
  9. 每管加入 2 ml 1× Permeabilization Buffer,室温下 300 - 400 × g 离心 5 分钟,弃去上清。
  10. (可选)重复步骤 9。
  11. 每管加入 500 μl 1× Flow Cytometry Staining Buffer (Cat No. S1001)重悬,上机检测。

实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

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