F11008A03

Anti-Human CD8α, APC (Clone: SK1) 流式抗体 (新品)

¥1,188.00 – ¥2,640.00

说明书

分子靶点：CD8A, CD8
种属：人 (Human)
标记：APC
亚型：Mouse IgG1, κ
克隆号：SK1

在售SKU:70-F11008A03-25, 70-F11008A03-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD8A 分子靶点信息概述
CD8A 分子靶点综述
- 人 Human CD8A 分子靶点信息
- 人 Human CD8A靶点分子功能（预测）

商品名	Anti-Human CD8α, APC (Clone: SK1) 流式抗体
靶点	CD8A, CD8
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	SK1
推荐应用	流式检测
用量	5 μL/Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.02% proclin950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD8α, APC (F11008A03)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD8A, CD8 靶点信息

CD8A 分子靶点信息概述

分子名：CD8A, CD8a molecule
基因家族：CD molecules; V-set domain containing
曾用名：CD8
全称：CD8 antigen, alpha polypeptide (p32); T-cell surface glycoprotein CD8 alpha chain

CD8A 分子靶点综述

CD8也称为T8或Leu2，是免疫球蛋白超家族的一员，表达于大部分胸腺细胞、外周血T细胞亚群和NK细胞(基本为CD8α/α同源二聚体)。CD8在抗原识别和T细胞活化中，作为MHC I类分子受限的T细胞受体，并在胸腺分化中具有重要作用。

人 Human CD8A 分子靶点信息

分子名：CD8A, CD8a molecule
别称：
- CD8
- cd8 antigen, alpha chain
- CD8 antigen, alpha polypeptide (p32)
- Cluster of Differentiation 8a
- Leu2
- Leu2 T-lymphocyte antigen
- MAL
- OKT8 T-cell antigen
- p32
- T cell co-receptor
- T-cell antigen Leu2
- T-cell surface glycoprotein CD8 alpha chain
- T-lymphocyte differentiation antigen T8/Leu-2
- T8 T-cell antigen
基因序列：NCBI_Gene: 925
蛋白序列：UniProtKB: P01732

人 Human CD8A靶点分子功能（预测）

Enables MHC class I protein complex binding activity. Predicted to be involved in T cell mediated immunity; cell surface receptor signaling pathway; and cytotoxic T cell differentiation. Predicted to act upstream of or within defense response to virus and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane and plasma membrane raft. Biomarker of COVID-19; erythema multiforme; and stomach cancer.

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD8α, APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD8α, APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 – 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。