EK204

Mouse IL-4 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥10,800.00

  • 分子靶点:IL4, IL-4, BSF1, BCGF1
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
  • 灵敏度:0.34 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:93% - 106%

在售SKU:70-EK204/2-48, 70-EK204/2-96

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描述

商品名

Mouse IL-4 ELISA Kit (小鼠白介素4 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.5% - 6.6%;板间变异系数:6.8% - 8.3%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:20 μL;细胞培养上清:100 μL

灵敏度

0.34 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

93% - 106%

平均回收率

1

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗 IL-4单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠 IL-4 冻干标准品
  • IL-4检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-4与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-4 ELISA Kit (小鼠白介素4 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL4, IL-4, BSF1, BCGF1 靶点信息

IL4 分子靶点信息概述

  • 分子名:IL4, interleukin 4
  • 基因家族:Interleukins
  • 别名:BSF1; IL-4; BCGF1; BCGF-1; MGC79402
  • 全称:B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4 分子靶点综述

IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子,在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡,促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态,研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用,对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用,并且IL-4对疫苗免疫应答具有调节作用。

小鼠 Mouse Il4 分子靶点信息

小鼠 Mouse Il4 靶点分子功能(预测)

Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren's syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).

操作步骤


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引用文献


  1. 支气管肺泡灌洗液 BALF 组织细胞

    Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

  2. 组织匀浆 肿瘤 血清

    An Injectable Hydrogel Reshaping Adenosinergic Axis for Cancer Therapy 

  3. 细胞培养上清 血清

    The Immunoenhancement Effects of Polyethylenimine-Modified Chinese Yam Polysaccharide-Encapsulated Plga Nanoparticles as an Adjuvant 

  4. 血清

    Vesicle-Mediated Dendritic Cell Activation in Acinetobacter Baumannii Clinical Isolate, Which Contributes to Th2 Response 

  5. Effects of Toll-Like Receptor 4 on Β2-Glycoprotein I-Induced Splenic T Cell Subsets Differentiation 

  6. The Combined Use of Cytokine-Induced Killer Cells and Cyclosporine a for the Treatment of Aplastic Anemia in a Mouse Model 

  7. Evaluation of Optimum Conditions for Achyranthes Bidentata Polysaccharides Encapsulated in Cubosomes and Immunological Activity in Vitro 

  8. In Vivo Mr Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with Nagdf(4) -Tat Nanoprobes 

  9. Reg3β from Cardiomyocytes Regulated Macrophage Migration, Proliferation and Functional Skewing in Experimental Autoimmune Myocarditis 

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