EK204

Mouse IL-4 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

分子靶点：IL4, IL-4, BSF1, BCGF1
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
灵敏度：0.34 pg/mL
检测范围：7.81 pg/mL - 500 pg/mL
回收率：93% - 106%

在售SKU:70-EK204/2-48, 70-EK204/2-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL4 分子靶点信息概述
IL4 分子靶点综述
- 小鼠 Mouse Il4 分子靶点信息
- 小鼠 Mouse Il4 靶点分子功能（预测）

商品名	Mouse IL-4 ELISA Kit (小鼠白介素4 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.5% - 6.6%；板间变异系数：6.8% - 8.3%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：20 μL；细胞培养上清：100 μL
灵敏度	0.34 pg/mL
检测范围	7.81 pg/mL - 500 pg/mL
回收率	93% - 106%
平均回收率	1
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗 IL-4单克隆抗体的96孔聚苯乙烯酶标板小鼠 IL-4 冻干标准品 IL-4检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×)

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-4与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-4 ELISA Kit (小鼠白介素4 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 IL4, IL-4, BSF1, BCGF1 靶点信息

IL4 分子靶点信息概述

分子名：IL4, interleukin 4
基因家族：Interleukins
别名：BSF1; IL-4; BCGF1; BCGF-1; MGC79402
全称：B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4 分子靶点综述

IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子，在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡，促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态，研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用，对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用，并且IL-4对疫苗免疫应答具有调节作用。

小鼠 Mouse Il4 分子靶点信息

分子名：Il4, interleukin 4
别称：
- Il-4
基因序列：NCBI_Gene: 16189
蛋白序列：

小鼠 Mouse Il4 靶点分子功能（预测）

Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren's syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).