EK1209

Human CX3CL1/Fractalkine ELISA Kit检测试剂盒(酶联免疫吸附法)

X

¥1,600.00¥2,650.00

  • 分子靶点:CX3CL1, NTN, CXC3
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:34.45 pg/mL
  • 检测范围:0.16 ng/mL - 10 ng/mL
  • 回收率:80% - 102%

在售SKU:70-EK1209-48, 70-EK1209-96

¥<\/span>1,600.00<\/bdi><\/span><\/span>","sku":"70-EK1209-48","variation_description":"","variation_id":9893,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":2650,"display_regular_price":2650,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>2,650.00<\/bdi><\/span><\/span>","sku":"70-EK1209-96","variation_description":"","variation_id":9894,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

Human CX3CL1/Fractalkine ELISA Kit (人趋化因子C-X3-C基序配体1 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.1% - 3.6%;板间变异系数:2.6% - 4.4%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

34.45 pg/mL

检测范围

0.16 ng/mL - 10 ng/mL

回收率

80% - 102%

平均回收率

0.88

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗CX3CL1单克隆抗体的96孔聚苯乙烯酶标板
  • 人CX3CL1冻干标准品
  • CX3CL1检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人 CX3CL1 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的 CX3CL1 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 CX3CL1 的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Human CX3CL1/Fractalkine ELISA Kit (人趋化因子C-X3-C基序配体1 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CX3CL1, NTN, CXC3 靶点信息

CX3CL1 分子靶点信息概述

  • 分子名:CX3CL1, C-X3-C motif chemokine ligand 1
  • 基因家族:Chemokine ligands
  • 别名:NTN; C3Xkine; ABCD-3; CXC3C; CXC3
  • 曾用名:SCYD1
  • 全称:fractalkine; neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); chemokine (C-X3-C motif) ligand 1

CX3CL1 分子靶点综述

趋化因子(C-X3-C基序)配体1(CX3CL1),又名fractalkine,是一个由373个氨基酸组成的大分子细胞因子蛋白,也是CX3C趋化因子家族中唯一一个已知成员。可溶性CX3CL1存在于血清、尿液、脑脊液、羊水和滑液中,主要由血管内皮、平滑肌、神经元和树突状细胞表达。炎性细胞因子如TNF-α和IFN-γ可诱导大多数CX3CL1的表达。CX3CL1是通过与趋化因子受体CX3CR1的相互作用来发挥其粘附和迁移功能。可溶性CX3XL1可强烈趋化T细胞和单核细胞,而细胞结合的趋化因子促进白细胞强有力粘附到活化的内皮细胞上。CX3CL1可能与抗肿瘤活性有关,在消退正常炎症过程如伤口愈合中有重要作用。CX3CL1对某些条件的发病具有促进作用。

人 Human CX3CL1 分子靶点信息

  • 分子名:CX3CL1, C-X3-C motif chemokine ligand 1
  • 别称:
    • ABCD-3
    • C-X3-C motif chemokine 1
    • C3Xkine
    • chemokine (C-X3-C motif) ligand 1
    • CX3C membrane-anchored chemokine
    • CXC3
    • CXC3C
    • fractalkine
    • neurotactin
    • NTN
    • NTT
    • SCYD1
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
    • small inducible cytokine subfamily D (Cys-X3-Cys), member-1
    • small-inducible cytokine D1
  • 基因序列:NCBI_Gene: 6376
  • 蛋白序列:UniProtKB: P78423

人 Human CX3CL1靶点分子功能(预测)

Enables signaling receptor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular region; and membrane. Is integral component of membrane. Implicated in Cor pulmonale and common cold. Biomarker of several diseases, including chronic fatigue syndrome; myositis (multiple); polyarteritis nodosa; pulmonary hypertension; and rheumatoid arthritis.

操作步骤


下载本产品说明书

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


评价

目前还没有评价

成为第一个“Human CX3CL1/Fractalkine ELISA Kit检测试剂盒(酶联免疫吸附法)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注