EK1202

Human Angiotensinogen/AGT/SerpinA8 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:AGT, angiotensinogen
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:19.93 pg/mL
  • 检测范围:0.31 ng/mL - 20 ng/mL
  • 回收率:83% - 105%

在售SKU:70-EK1202-48, 70-EK1202-96

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描述

商品名

Human Angiotensinogen/AGT/ SerpinA8 ELISA Kit (人血管紧张素原 (AGT) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.0% - 4.9%;板间变异系数:3.7%-4.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

19.93 pg/mL

检测范围

0.31 ng/mL - 20 ng/mL

回收率

83% - 105%

平均回收率

0.96

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Angiotensinogen/AGT/ SerpinA8单克隆抗体的96孔聚苯乙烯酶标板
  • 人Angiotensinogen/AGT/ SerpinA8冻干标准品
  • Angiotensinogen/AGT/ SerpinA8检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人AGT抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的AGT与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中AGT的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Angiotensinogen/AGT/ SerpinA8 ELISA Kit (人血管紧张素原 (AGT) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 AGT, angiotensinogen 靶点信息

AGT 分子靶点信息概述

  • 分子名:AGT, angiotensinogen
  • 基因家族:Serpin peptidase inhibitors; Neuropeptides
  • 曾用名:SERPINA8
  • 全称:alpha-1 antiproteinase, antitrypsin; serpin peptidase inhibitor, clade A, member 8

AGT 分子靶点综述

血管紧张素原(AGT),又称为SerpinA8,是丝氨酸蛋白酶抑制剂家族的一员。AGT是一个α-2-球蛋白,由肝脏组成性产生并释放到血液循环中。AGT是肾素-血管紧张素系统的一个必不可少的组分,是一个有效的血压调节剂。AGT可裂解成3条链:血管紧张素-1、血管紧张素-2和血管紧张素-3。AGT的缺失与原发性高血压及肾小管发育不全相关。一些丝氨酸蛋白酶抑制剂(抗凝血酶、maspin、色素上皮衍生因子和kallistatin)最近已被证明具有抗血管生成活性,表明内皮细胞增殖和迁移的共同机制。AGT和它的肾素裂解产物,在体外和体内可观察到的血浆浓度范围内,也是血管生成抑制剂。

人 Human AGT 分子靶点信息

  • 分子名:AGT, angiotensinogen
  • 别称:
    • alpha-1 antiproteinase, antitrypsin
    • angiotensin I
    • angiotensin II
    • angiotensinogen (serine (or cysteine) proteinase inhibitor, clade a (alpha-1 antiproteinase, antitrypsin), member 8)
    • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
    • ANHU
    • fetal-liver predominant transporter 1
    • FLJ92595
    • FLJ97926
    • hFLT1
    • pre-angiotensinogen
    • serine (or cysteine) proteinase inhibitor
    • serpin A8
    • serpin peptidase inhibitor, clade A, member 8
    • SERPINA8
  • 基因序列:NCBI_Gene: 183
  • 蛋白序列:UniProtKB: P01019

人 Human AGT靶点分子功能(预测)

Enables angiotensin receptor binding activity; receptor ligand activity; and sodium channel regulator activity. Involved in several processes, including positive regulation of peptidyl-tyrosine phosphorylation; regulation of gene expression; and regulation of signal transduction. Located in extracellular space. Part of collagen-containing extracellular matrix. Implicated in several diseases, including Fabry disease; Henoch-Schoenlein purpura; artery disease (multiple); heart conduction disease (multiple); and hydronephrosis. Biomarker of several diseases, including alcoholic hepatitis; chronic myeloid leukemia; intermediate coronary syndrome; systemic scleroderma (multiple); and type 2 diabetes mellitus.

操作步骤


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