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KTR201

Mouse Regulatory T Cell Staining Kit小鼠调节性T细胞染色试剂盒

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¥1,490.00 – ¥4,570.00

在售SKU:70-KTR201-100, 70-KTR201-25,小鼠,mouse,Treg,Tregs,调节性T细胞,调节,T细胞,调节T细胞

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描述

产品简介

调节性T细胞(Tregs)，曾被称为抑制性T细胞，是T细胞的亚群，可调节免疫系统、维持对自身抗原的耐受、抑制自身免疫疾病。Tregs具有免疫抑制性，可抑制或下调效应T细胞的诱导和增殖。调节性T细胞有多种类型，其中研究最深的的一类表达CD4、CD25和FOXP3(CD4+CD25+调节性T细胞)。免疫系统必须能区分自我和非我。当自我/非我无法识别时，免疫系统会破坏细胞和组织，引起自身免疫疾病。调节性T细胞积极地抑制免疫系统活化、阻止病理性的自身反应如自身免疫疾病。调节性T细胞遗传缺失造成的重度自身免疫综合征(IPEX综合征)表明调节性T细胞在免疫系统中发挥了重要作用。

产品特点

  • 即用型，无需购买其他产品；
  • 灵敏度高，特异性强，结果稳定；
  • 价格便宜，现货供应。

结果示例

Flow cytometric analysis of Mouse Regulatory T Cell Staining Kit. The staining pattern of FSC/CD4 (Left) CD25/Mouse IgG1 Isotype Control, PE (Middle) and CD25/FoxP3 (Right) on splenocytes of normal ICR mouse. Adherent cells have been excluded. Flow cytometry was performed on a Thermo Fisher Attune NxT.

使用Mouse Regulatory T Cell Staining Kit进行流式检测。正常ICR小鼠的脾细胞染色FSC/CD4 (左) CD25/小鼠IgG1同型对照, PE (中)和CD25/FoxP3(右)。粘连细胞已被排除。实验在Thermo Fisher公司的Attune NxT流式细胞仪上进行。

试剂盒组分

组分 编号 25Test 100Test
抗小鼠CD4, FITC (克隆号：GK1.5) AM00401 150 μl 600 μl
抗小鼠 CD25, APC (克隆号：PC61.5) AM02505 150 μl 600 μl
抗小鼠FoxP3, PE (克隆号：3G3) AM0F04 150 μl 600 μl
抗小鼠CD16/CD32, 纯化 (克隆号：2.4G2) AM016 60 μl 240 μl
小鼠IgG1同型对照, PE CMG104 25 μl 100 μl
固定破膜剂浓缩液(4×) ICC01 8 ml 30 ml
固定破膜剂稀释液 ICD01 25 ml 100 ml
破膜缓冲液(10×) ICB01 25 ml 100 ml
红细胞裂解液(不含固定剂) (10×) LYS 8 ml 30 ml
流式染色缓冲液(10×) S1002 15 ml 60 ml

引用文献

  1. Fecal microbiota transplantation inhibited neuroinflammation of traumatic brain injury in mice via regulating the gut-brain axis 

  2. TIL 肿瘤浸润淋巴细胞 血清

    Elastic Nanovaccine Enhances Dendritic Cell-Mediated Tumor Immunotherapy 

Shao, B., Huang, X., Xu, F. et al. A pH-responsive polymersome depleting regulatory T cells and blocking A2A receptor for cancer immunotherapy. Nano Res. 15, 2324–2334 (2022).（IF=8.896）

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