EK1152

Human Thrombopoietin/TPO ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:THPO, TPO
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:11.32 pg/mL
  • 检测范围:62.5 pg/mL - 4000 pg/mL
  • 回收率:72% - 124%

在售SKU:70-EK1152-48, 70-EK1152-96

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描述

商品名

Human Thrombopoietin/TPO ELISA Kit (人促血小板生成素 (TPO) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.6% - 6.4%;板间变异系数:2.3% - 5.4%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

11.32 pg/mL

检测范围

62.5 pg/mL - 4000 pg/mL

回收率

72% - 124%

平均回收率

0.97

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Thrombopoietin/TPO单克隆抗体的96孔聚苯乙烯酶标板
  • 人Thrombopoietin/TPO冻干标准品
  • Thrombopoietin/TPO检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TPO抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TPO与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TPO的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Thrombopoietin/TPO ELISA Kit (人促血小板生成素 (TPO) ELISA试剂盒) - 标准曲线
标准曲线

操作步骤


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