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KTH201

Mouse Th1/Th2 Staining Kit 小鼠辅助性Th1/Th2细胞检测试剂盒

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¥1,850.00 – ¥6,940.00

在售SKU:70-KTH201-100, 70-KTH201-25, th1, th2, 染色, Th, 分型, 细胞分型, 免疫分型, 检测, 分型检测, 试剂盒

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描述

产品简介

本产品用于检测小鼠抗凝血或脾细胞中，Th1和Th2细胞在T淋巴细胞 (或CD4+辅助T细胞)中的比例。通常所说的Th1、Th2和Th17是指在各种生理与病理条件下有能力分化为Th1、Th2和Th17 的T辅助细胞 (严格意义上是Th0细胞)。静息状态 (即未受任何刺激，如人的正常生理状态)下，Th0分化为Th1、Th2和Th17的能力非常弱，所以外周血中仅含有极少量的Th1、Th2和Th17细胞，这时所能检测到的IFN-γ、IL-4和IL-17A也微乎其微。而当Th细胞受到外界因素 (如刺激素、病原体等)刺激，其中Th0即会向Th1、Th2或Th17分化，具体分化趋向取决于细胞因子的种类。此时，检测到的IFN-γ、IL-4或IL-17A也较多。实验中检测的Th1、Th2和Th17实际上是检测Th细胞对刺激素刺激的反应能力。

产品特点

  • 即用型，无需购买其他产品；
  • 灵敏度高，特异性强，结果稳定；
  • 价格便宜，现货供应。

结果示例


Flow cytometric analysis of Mouse Th1/Th2 Staining Kit. The staining pattern of IFN-γ and IL-4 on resting heparin anticoagulated blood (A0, B0), PBMCs from EDTA anticoagulated blood (C0, D0), splenocytes (E0, F0) and spleen mononuclear cells (G0, H0) of normal ICR mouse. PMA/Ionomycin stimulated heparin anticoagulated blood (A1, B1), PBMCs from EDTA anticoagulated blood (C1, D1), splenocytes (E1, F1) and spleen mononuclear cells (G1, H1) of normal ICR mouse. Dot plot analysis are derived from gated CD3+/CD4+ cells population. Flow cytometry was performed on a Thermo Fisher Attune NxT.

使用Mouse Th1/Th2 Staining Kit进行流式检测。正常ICR小鼠的静息肝素抗凝血(A0, B0)、EDTA抗凝血来源的PBMCs (C0, D0)、脾细胞(E0, F0)和脾单个核细胞(G0, H0)染色IFN-γ和IL-4。正常ICR小鼠的PMA/Ionomycin刺激的肝素抗凝血(A1, B1)、EDTA抗凝血来源的PBMCs (C1, D1) 脾细胞(E1, F1)和脾单个核细胞(G1, H1)染色IFN-γ和IL-4。对CD3+/CD4+细胞进行设门分析。实验在Thermo Fisher公司的Attune NxT流式细胞仪上进行。

试剂盒组分

组分 编号 25Test 100Test
抗小鼠CD3ε, FITC (克隆号：145-2C11) AM003E01 150 μl 600 μl
抗小鼠CD4, PerCP- Cy5.5 (克隆号：GK1.5) AM00407 150 μl 600 μl
抗小鼠IFN-γ, PE (克隆号：XMG1.2) AM0IF04 150 μl 600 μl
抗小鼠IL-4, APC (克隆号：11B11) AM0I405 150 μl 600 μl
佛波酯/离子霉素混合物(250×) CS1001 100 μl 200 μl
布雷非德菌素A/莫能霉素混合物(250×) CS1002 100 μl 200 μl
固定破膜剂试剂A GASA 3 ml 11 ml
固定破膜剂试剂B GASB 3 ml 11 ml
流式染色缓冲液 (10×) S1002 15 ml 60 ml

引用文献

Zhenxing Chen,Qiangqiang YanImmunomodulatory Effects of Hydrolyzed Seawater Pearl Tablet(HSPT) on Th1/Th2 Functionality in a Mice Model of Chronic Obstructive Pulmonary Disease (COPD) Induced by Cigarette Smoke

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