EK202

Mouse IL-2 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:IL2, IL-2
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
  • 灵敏度:0.54 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:93% - 113%

在售SKU:70-EK202/2-48, 70-EK202/2-96

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描述

商品名

Mouse IL-2 ELISA Kit (小鼠白介素2 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.3% - 6.0%;板间变异系数:5.1% - 9.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:20 μL;细胞培养上清:100 μL

灵敏度

0.54 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

93% - 113%

平均回收率

1

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗 IL-2单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠 IL-2 冻干标准品
  • IL-2检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-2 ELISA Kit (小鼠白介素2 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

  • 分子名:IL2, interleukin 2
  • 基因家族:Interleukins
  • 别名:IL-2; TCGF
  • 全称:T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白,在人类中,由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子,调控对具有免疫功能的白血球(白细胞,通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答,区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能,其受体由3条链组成,分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角,包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

小鼠 Mouse Il2 分子靶点信息

小鼠 Mouse Il2 靶点分子功能(预测)

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in negative regulation of T-helper 17 cell differentiation. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway in absence of ligand; positive regulation of macromolecule metabolic process; and regulation of lymphocyte activation. Located in extracellular space. Is expressed in liver; omental bursa; thymus; and thymus primordium. Used to study Sjogren's syndrome and inflammatory bowel disease. Human ortholog(s) of this gene implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Orthologous to human IL2 (interleukin 2).

操作步骤


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