EK304HS

Rat IL-4 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL4, IL-4, BSF1, BCGF1
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.20 pg/mL
检测范围：3.13 pg/mL - 200 pg/mL
回收率：81% - 101%

在售SKU:70-EK304HS-48, 70-EK304HS-96

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库存号

描述

文章目录[隐藏]

分子信息
IL4 分子靶点信息概述
IL4 分子靶点综述
- 大鼠 Rat Il4 分子靶点信息
- 大鼠 Rat Il4 靶点分子功能（预测）

商品名	Rat IL-4 High Sensitivity ELISA Kit (大鼠白细胞介素4高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：5.0% - 6.8%；板间变异系数：2.7% - 7.5%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.20 pg/mL
检测范围	3.13 pg/mL - 200 pg/mL
回收率	81% - 101%
平均回收率	0.9
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-4单克隆抗体的96孔聚苯乙烯酶标板大鼠IL-4冻干标准品 IL-4检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-4与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-4 High Sensitivity ELISA Kit (大鼠白细胞介素4高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IL4, IL-4, BSF1, BCGF1 靶点信息

IL4 分子靶点信息概述

分子名：IL4, interleukin 4
基因家族：Interleukins
别名：BSF1; IL-4; BCGF1; BCGF-1; MGC79402
全称：B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4 分子靶点综述

IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子，在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡，促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态，研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用，对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用，并且IL-4对疫苗免疫应答具有调节作用。

大鼠 Rat Il4 分子靶点信息

分子名：Il4, interleukin 4
别称：
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
基因序列：NCBI_Gene: 287287
蛋白序列：
- UniprotKB: A0A0G2JVY1
- UniprotKB: P20096

大鼠 Rat Il4 靶点分子功能（预测）

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).