EK304HS

Rat IL-4 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:IL4, IL-4, BSF1, BCGF1
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:0.20 pg/mL
  • 检测范围:3.13 pg/mL - 200 pg/mL
  • 回收率:81% - 101%

在售SKU:70-EK304HS-48, 70-EK304HS-96

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描述

商品名

Rat IL-4 High Sensitivity ELISA Kit (大鼠白细胞介素4高敏 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:5.0% - 6.8%;板间变异系数:2.7% - 7.5%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

0.20 pg/mL

检测范围

3.13 pg/mL - 200 pg/mL

回收率

81% - 101%

平均回收率

0.9

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-4单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-4冻干标准品
  • IL-4检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-4与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-4 High Sensitivity ELISA Kit (大鼠白细胞介素4高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL4, IL-4, BSF1, BCGF1 靶点信息

IL4 分子靶点信息概述

  • 分子名:IL4, interleukin 4
  • 基因家族:Interleukins
  • 别名:BSF1; IL-4; BCGF1; BCGF-1; MGC79402
  • 全称:B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1

IL4 分子靶点综述

IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子,在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡,促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态,研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用,对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用,并且IL-4对疫苗免疫应答具有调节作用。

大鼠 Rat Il4 分子靶点信息

  • 分子名:Il4, interleukin 4
  • 别称:
    • B-cell growth factor 1
    • B-cell IGG differentiation factor
    • B-cell stimulatory factor 1
    • BSF-1
    • IL-4
    • Il4e12
    • interleukin-4
    • lymphocyte stimulatory factor 1
  • 基因序列:NCBI_Gene: 287287
  • 蛋白序列:

大鼠 Rat Il4 靶点分子功能(预测)

Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).

操作步骤


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