EK106HS

Human IL-6 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL6, IL-6, IFNB2, HGF
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.02 pg/mL
检测范围：0.31 pg/mL - 20 pg/mL
回收率：74% - 119%

在售SKU:70-EK106HS-48, 70-EK106HS-96, 人白细胞介素6, 白细胞介素6, 白细胞介素, 人, 白介素, EK106HS

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL6 分子靶点信息概述
IL6 分子靶点综述
- 人 Human IL6 分子靶点信息
- 人 Human IL6靶点分子功能（预测）

商品名	Human IL-6 High Sensitivity ELISA Kit (人白介素6高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.7% - 5.6%；板间变异系数：4.6% - 6.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.02 pg/mL
检测范围	0.31 pg/mL - 20 pg/mL
回收率	74% - 119%
平均回收率	0.94
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板人IL-6冻干标准品 IL-6检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-6 High Sensitivity ELISA Kit (人白介素6高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

分子名：IL6, interleukin 6
基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
别名：IL-6; BSF2; HGF; HSF
曾用名：IFNB2
全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

人 Human IL6 分子靶点信息

分子名：IL6, interleukin 6
别称：
- B cell stimulatory factor-2
- B-cell differentiation factor
- B-cell stimulatory factor 2
- BSF-2
- BSF2
- CDF
- CTL differentiation factor
- HGF
- HSF
- hybridoma growth factor
- IFN-beta-2
- IFNB2
- IL-6
- interferon beta-2
- interferon, beta 2
- interleukin BSF-2
- interleukin-6
基因序列：NCBI_Gene: 3569
蛋白序列：UniProtKB: P05231

人 Human IL6靶点分子功能（预测）

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).