EK107

Human IL-7 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:IL7, IL-7
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:血清血浆:20 μL;细胞培养上清:100 μL
  • 灵敏度:0.22 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:80% - 115%

在售SKU:70-EK107-48, 70-EK107-96, 人白细胞介素7, 白细胞介素7, 白细胞介素, 人, 白介素, 7

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描述

商品名

Human IL-7 ELISA Kit (人白细胞介素7ELISA 试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.5% - 5.5%;板间变异系数:2.7% - 3.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

血清血浆:20 μL;细胞培养上清:100 μL

灵敏度

0.22 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

80% - 115%

平均回收率

1.01

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-7单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-7冻干标准品
  • IL-7 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-7抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-7与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-7的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-7 ELISA Kit (人白细胞介素7ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL7, IL-7 靶点信息

IL7 分子靶点信息概述

  • 分子名:IL7, interleukin 7
  • 基因家族:Interleukins
  • 别名:IL-7

IL7 分子靶点综述

白细胞介素7(IL-7)是一种由骨髓和胸腺基质细胞分泌产生的造血生长因子,它也可由角质形成细胞、树突状细胞、肝细胞、神经元和上皮细胞产生,但是正常的淋巴细胞不能产生IL-7。IL-7刺激多能造血干细胞向淋巴系祖细胞的分化,同样也可刺激所有淋巴系细胞(B细胞、T细胞和NK细胞)的增殖。IL-7促进恶性血液病(急性淋巴细胞白血病、T细胞淋巴瘤)的发展,升高的IL-7水平在HIV感染患者血浆中被检测到,IL-7也可提高异体干细胞移植后的免疫康复。

人 Human IL7 分子靶点信息

人 Human IL7靶点分子功能(预测)

Enables cytokine activity. Involved in cytokine-mediated signaling pathway; positive regulation of chemokine production; and positive regulation of cytokine-mediated signaling pathway. Located in extracellular region. Part of collagen-containing extracellular matrix. Implicated in epidermodysplasia verruciformis. Biomarker of Alzheimer's disease and COVID-19.

操作步骤


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