EK101A

Human IL-1a ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:IL1A, IL1-α, IL1F1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL 1×检测缓冲液和50 μL样本
  • 灵敏度:0.52 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:81% - 106%

在售SKU:70-EK101A-48, 70-EK101A-96, 人白介素1α, 白介素1α, 白介素1A, 人, 白介素, 1, α, alpha

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描述

商品名

Human IL-1α ELISA Kit (人白介素1α ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 4.4%;板间变异系数:3.9% - 6.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL 1×检测缓冲液和50 μL样本

灵敏度

0.52 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

81% - 106%

平均回收率

0.93

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-1α单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-1α冻干标准品
  • IL-1α检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-1α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-1α与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-1α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-1α ELISA Kit (人白介素1α ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL1A, IL1-α, IL1F1 靶点信息

IL1A 分子靶点信息概述

  • 分子名:IL1A, interleukin 1 alpha
  • 基因家族:Interleukins
  • 别名:IL1F1; IL-1A; IL1-ALPHA
  • 曾用名:IL1
  • 全称:preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha

IL1A 分子靶点综述

白细胞介素1(IL-1)是17kDa的胞外多肽,分为IL-1α和IL-1β两种蛋白。IL-1α是一种多效性细胞因子,在人类中由IL1A基因编码。IL-1α主要由活化的巨噬细胞、中性粒细胞、上皮细胞和内皮细胞产生。它具有代谢、生理、造血活性,在调节免疫应答中起到核心作用。IL-1α可与HAX1、NDN相互作用,但是与TNF之间的协同作用是临床上最相关的。IL-1α和TNF都是急性期细胞因子,具有促发热和发炎作用。人们目前正在评估IL-1α是否可作为临床试验中一个潜在治疗肿瘤的标志物。阻断IL-1α活性具有潜在治疗皮肤病的作用,例如痤疮。

人 Human IL1A 分子靶点信息

  • 分子名:IL1A, interleukin 1 alpha
  • 别称:
    • hematopoietin-1
    • IL-1 alpha
    • IL-1A
    • IL1
    • IL1-ALPHA
    • IL1F1
    • interleukin 1, alpha
    • interleukin-1 alpha
    • preinterleukin 1 alpha
    • pro-interleukin-1-alpha
  • 基因序列:NCBI_Gene: 3552
  • 蛋白序列:UniProtKB: P01583

人 Human IL1A靶点分子功能(预测)

Enables copper ion binding activity and cytokine activity. Involved in several processes, including positive regulation of interleukin-2 production; positive regulation of mitotic nuclear division; and response to copper ion. Located in cytosol and extracellular space. Implicated in several diseases, including Fabry disease; Schnitzler syndrome; autoimmune disease (multiple); hematologic cancer (multiple); and lung disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); cholesteatoma (multiple); common cold; cystic fibrosis; and osteoarthritis.

操作步骤


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