EK206

Mouse IL-6 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

分子靶点：IL6, IL-6, IFNB2, HGF
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：10 μL
灵敏度：0.56 pg/mL
检测范围：7.81 pg/mL - 500 pg/mL
回收率：89% - 112%

在售SKU:70-EK206/3-48, 70-EK206/3-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
IL6 分子靶点信息概述
IL6 分子靶点综述
- 小鼠 Mouse Il6 分子靶点信息
- 小鼠 Mouse Il6 靶点分子功能（预测）

商品名	Mouse IL-6 ELISA Kit (小鼠白细胞介素6 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.3% - 3.0%；板间变异系数：2.1% - 5.3%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	10 μL
灵敏度	0.56 pg/mL
检测范围	7.81 pg/mL - 500 pg/mL
回收率	89% - 112%
平均回收率	1.02
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-6单克隆抗体的96孔聚苯乙烯酶标板小鼠IL-6冻干标准品 IL-6检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-6 ELISA Kit (小鼠白细胞介素6 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

分子名：IL6, interleukin 6
基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
别名：IL-6; BSF2; HGF; HSF
曾用名：IFNB2
全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

小鼠 Mouse Il6 分子靶点信息

小鼠 Mouse Il6 靶点分子功能（预测）

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).