EK382EGA

EasyGo!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,190.00¥3,650.00

  • 分子靶点:TNF, TNFA, TNFSF2
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆
  • 检测样本体积:10 μL
  • 灵敏度:3.18 pg/mL
  • 检测范围:4.69 pg/mL - 300 pg/mL
  • 回收率:77% - 122%

在售SKU:70-EK382EG-48, 70-EK382EG-96, EK382EG

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描述

商品名

EasyGo!™ Rat TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:8.4% - 9.5%;板间变异系数:5.4% - 9.0%

样本类型

血清,血浆

检测样本体积

10 μL

灵敏度

3.18 pg/mL

检测范围

4.69 pg/mL - 300 pg/mL

回收率

77% - 122%

平均回收率

0.95

板式

96孔板,可拆

保存

试剂盒未拆开,2 - 8℃保存。已拆开,标准品-20℃保存,其它2 - 8℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 5630 nm)测定吸光度值。

EasyGo!™ Rat TNF-α One-Step ELISA Kit EasyGo!™ 大鼠肿瘤坏死因子α (TNF-α) 一步法酶联免疫吸附测定试剂盒 - 标准曲线
标准曲线

分子信息

概述大鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

大鼠 Rat Tnf 分子靶点信息

  • 分子名:Tnf, tumor necrosis factor
  • 别称:
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • 基因序列:NCBI_Gene: 24835
  • 蛋白序列:UniProtKB: P16599

大鼠 Rat Tnf 靶点分子功能(预测)

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

操作步骤


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