EK182EG

EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,190.00 – ¥3,650.00

说明书

分子靶点：TNF, TNFA, TNFSF2
种属：人 (Human)
样本类型：血清，血浆
检测样本体积：10 μL
灵敏度：1.77 pg/mL
检测范围：3.13 pg/mL - 200 pg/mL
回收率：100% - 122%

在售SKU: 70-EK182EGA-48, 70-EK182EGA-96, EK182EGA, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

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Size
库存号

描述

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 小鼠 Mouse Tnf 分子靶点信息
- 小鼠 Mouse Tnf 靶点分子功能（预测）

商品名	EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	一步法
精密度	板内变异系数：4.2% - 5.4%；板间变异系数：5.5% - 6.2%
样本类型	血清，血浆
检测样本体积	10 μL
灵敏度	1.77 pg/mL
检测范围	3.13 pg/mL - 200 pg/mL
回收率	100% - 122%
平均回收率	1.08
板式	96孔板，可拆
保存	试剂盒未拆开，2-8℃保存。已拆开，标准品-20℃保存，其它2-8℃保存。
运输条件	4℃蓝冰运输
组分	含微球的预包被酶标板标准品标准品稀释液显色底物终止液 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒（酶联免疫吸附法）- 标准曲线 — 标准曲线

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

肿瘤坏死因子α (TNF-α)，也称为恶病质素和 TNFSF1A，是一种脂肪因子，参与全身的炎症，同时也是刺激急性期反应的细胞因子之一。它主要由活化的巨噬细胞产生，也可由其它类型的细胞分泌，如 CD4+淋巴细胞、NK 细胞、中性粒细胞、肥大细胞、嗜酸性粒细胞和神经元等。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物 (感染因子或组织受损) 产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α在关节组织和其它组织发生炎症的发病机制具有重要作用。最近，越来越多的信息表明 TNF-α也参与了 AIDS 的发病机制。TNF-α 的测定对于移植研究也非常有用。

小鼠 Mouse Tnf 分子靶点信息

分子名：Tnf, tumor necrosis factor
别称：
- DIF
- TNF alpha
- TNF-alpha
- Tnfa
- TNFalpha
- Tnfsf1a
- tumor necrosis factor-alpha
- tumor necrosis factor, alpha
基因序列：NCBI_Gene: 21926
蛋白序列：

小鼠 Mouse Tnf 靶点分子功能（预测）

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).