EK182EG

EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,190.00¥3,650.00

  • 分子靶点:TNF, TNFA, TNFSF2
  • 种属:人 (Human)
  • 样本类型:血清,血浆
  • 检测样本体积:10 μL
  • 灵敏度:1.77 pg/mL
  • 检测范围:3.13 pg/mL - 200 pg/mL
  • 回收率:100% - 122%

在售SKU: 70-EK182EGA-48, 70-EK182EGA-96, EK182EGA, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

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描述

商品名

EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

一步法

精密度

板内变异系数:4.2% - 5.4%;板间变异系数:5.5% - 6.2%

样本类型

血清,血浆

检测样本体积

10 μL

灵敏度

1.77 pg/mL

检测范围

3.13 pg/mL - 200 pg/mL

回收率

100% - 122%

平均回收率

1.08

板式

96孔板,可拆

保存

试剂盒未拆开,2-8℃保存。已拆开,标准品-20℃保存,其它2-8℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™Human TNF-α One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)- 标准曲线
标准曲线

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

肿瘤坏死因子α (TNF-α),也称为恶病质素和 TNFSF1A,是一种脂肪因子,参与全身的炎症,同时也是刺激急性期反应的细胞因子之一。它主要由活化的巨噬细胞产生,也可由其它类型的细胞分泌,如 CD4+淋巴细胞、NK 细胞、中性粒细胞、肥大细胞、嗜酸性粒细胞和神经元等。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物 (感染因子或组织受损) 产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α在关节组织和其它组织发生炎症的发病机制具有重要作用。最近,越来越多的信息表明 TNF-α也参与了 AIDS 的发病机制。TNF-α 的测定对于移植研究也非常有用。

小鼠 Mouse Tnf 分子靶点信息

小鼠 Mouse Tnf 靶点分子功能(预测)

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

操作步骤

检验步骤概要

  1. 1)标准品孔加入100 μL 2倍倍比稀释的标准品。空白孔加入100 μL 标准品稀释液或培养基。
  2. 2)血清/血浆:样本孔加入90 μL 标准品稀释液和10 μL样本。细胞培养上清:样本孔加入33 μL细胞培养上清和67μL标准品稀释液。
  3. 3)37℃孵育1小时,或室温(25℃±3℃)孵育2小时。撕膜,洗涤3次。
  4. 每孔加入 100 μL 显色底物,避光,室温(25℃±3℃)孵育 10±5 分钟。
  5. 每孔加入 100 μL 终止液。
  6. 30 分钟内,在 450nm 波长检测 OD 值,参考波长 570nm 或 630nm。

检测步骤简略图示

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