EK108HS

Human IL-8 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：CXCL8, IL-8, GCP1, NAP1
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：50 μL；细胞培养上清：100 μL
灵敏度：0.28 pg/mL
检测范围：2.34 pg/mL - 150 pg/mL
回收率：91% - 114%

在售SKU:70-EK108HS-48, 70-EK108HS-96, 人白细胞介素8, 白细胞介素8, 白细胞介素, 人, 白介素, 8

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库存号

描述

文章目录[隐藏]

分子信息
CXCL8 分子靶点信息概述
CXCL8 分子靶点综述
- 人 Human CXCL8 分子靶点信息
- 人 Human CXCL8靶点分子功能（预测）

商品名	Human IL-8 High Sensitivity ELISA Kit (人白细胞介素8 高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.3% - 5.0%；板间变异系数：3.3% - 4.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：50 μL；细胞培养上清：100 μL
灵敏度	0.28 pg/mL
检测范围	2.34 pg/mL - 150 pg/mL
回收率	91% - 114%
平均回收率	1.02
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-8 单克隆抗体的96孔聚苯乙烯酶标板人IL-8 高敏冻干标准品 IL-8检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-8抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-8与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-8的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-8 High Sensitivity ELISA Kit (人白细胞介素8 高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 CXCL8, IL-8, GCP1, NAP1 靶点信息

CXCL8 分子靶点信息概述

分子名：CXCL8, C-X-C motif chemokine ligand 8
基因家族：Chemokine ligands; Interleukins
别名：SCYB8; LUCT; LECT; MDNCF; TSG-1; IL-8; NAP-1; 3-10C; MONAP; AMCF-I; LYNAP; NAF; b-ENAP; GCP-1; K60; GCP1; NAP1
曾用名：IL8
全称：neutrophil-activating peptide 1; granulocyte chemotactic protein 1; monocyte-derived neutrophil chemotactic factor; lung giant cell carcinoma-derived chemotactic protein; tumor necrosis factor-induced gene 1; monocyte-derived neutrophil-activating peptide; lymphocyte derived neutrophil activating peptide; beta endothelial cell-derived neutrophil activating peptide; alveolar macrophage chemotactic factor I; interleukin 8; chemokine (C-X-C motif) ligand 8

CXCL8 分子靶点综述

白细胞介素8(IL-8)是由巨噬细胞和其它类型细胞如上皮细胞、气道平滑肌细胞和内皮细胞产生的趋化因子，在人类中，由IL8基因编码。IL-8可有效促进血管生成，在细支气管炎的发病机制中起重要作用。IL-8可通过对中性粒细胞的召集和脱颗粒来实现对炎症反应的调节，可作为结肠癌细胞株的自分泌生长因子来作用于结肠直肠癌。高水平的IL-8可减少精神分裂症患者抗精神病药的阳性反应。另外，IL-8与肥胖和囊性纤维化的发病机制也相关。

人 Human CXCL8 分子靶点信息

分子名：CXCL8, C-X-C motif chemokine ligand 8
别称：
- alveolar macrophage chemotactic factor I
- beta endothelial cell-derived neutrophil activating peptide
- beta-thromboglobulin-like protein
- C-X-C motif chemokine 8
- chemokine (C-X-C motif) ligand 8
- emoctakin
- GCP-1
- GCP1
- granulocyte chemotactic protein 1
- IL8
- interleukin 8
- interleukin-8
- LECT
- LUCT
- lung giant cell carcinoma-derived chemotactic protein
- lymphocyte derived neutrophil activating peptide
- lymphocyte-derived neutrophil-activating factor
- LYNAP
- MDNCF
- MONAP
- monocyte-derived neutrophil chemotactic factor
- monocyte-derived neutrophil-activating peptide
- NAF
- NAP-1
- NAP1
- neutrophil-activating peptide 1
- SCYB8
- small inducible cytokine subfamily B, member 8
- T cell chemotactic factor
- T-cell chemotactic factor
- tumor necrosis factor-induced gene 1
基因序列：NCBI_Gene: 3576
蛋白序列：UniProtKB: P10145

人 Human CXCL8靶点分子功能（预测）

Enables chemokine activity and interleukin-8 receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; induction of positive chemotaxis; and neutrophil chemotaxis. Acts upstream of or within negative regulation of cell adhesion molecule production; positive regulation of cellular biosynthetic process; and regulation of gene expression. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in several diseases, including autoimmune uveitis; bacterial gastritis; gastrointestinal system cancer (multiple); trachoma; and urinary tract infection. Biomarker of several diseases, including chikungunya; cholestasis (multiple); cystic fibrosis; liver disease (multiple); and tuberculosis (multiple).