EK406

Guinea pig IL-6 ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL6, IL-6, IFNB2, HGF
种属：豚鼠 (Guinea pig)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：1.74 pg/mL
检测范围：7.813 pg/mL - 500 pg/mL
回收率：83% - 118%

在售SKU:70-EK406-48, 70-EK406-96, EK406

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库存号

描述

文章目录[隐藏]

分子信息
IL6 分子靶点信息概述
IL6 分子靶点综述
- 豚鼠 Guinea pig Il6 分子靶点信息

商品名	Guinea pig IL-6 ELISA Kit (豚鼠白细胞介素6 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.3% - 6.8%；板间变异系数：5.9% - 9.6%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	1.74 pg/mL
检测范围	7.813 pg/mL - 500 pg/mL
回收率	83% - 118%
平均回收率	1.07
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗豚鼠白介素 6捕获抗体的96孔聚苯乙烯酶标板 Guinea Pig IL-6冻干标准品 Guinea Pig IL-6检测抗体 Streptavidin-HRP 检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜标准品稀释液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗豚鼠IL-6抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-6与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-6的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Guinea pig IL-6 ELISA Kit 检测试剂盒 - 标准曲线 — 标准曲线

分子信息

概述豚鼠 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

分子名：IL6, interleukin 6
基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
别名：IL-6; BSF2; HGF; HSF
曾用名：IFNB2
全称：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中，它由IL-6基因编码。在感染期间和受到外伤后，尤其是烧伤或其它组织损伤导致的炎症，IL-6会由T细胞和巨噬细胞分泌，促进免疫应答。研究表明，小鼠抵抗肺炎链球菌感染时需要IL-6的参与，说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。