F110I205

Anti-Human IL-2, PE-Cy7 (Clone: MQ1-17H12) 检测试剂

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¥1,470.00¥3,340.00

  • 分子靶点:IL2, IL-2
  • 种属:人 (Human)
  • 标记:PE-Cy7
  • 亚型:Rat IgG2a, κ
  • 克隆号:MQ1-17H12

在售SKU,70-F110I205-25, 70-F110I205-100

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描述

商品名

Anti-Human IL-2, PE-Cy7 (Clone: MQ1-17H12) 检测试剂

靶点

IL2, IL-2

标记

PE-Cy7

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Rat IgG2a, κ

克隆号

MQ1-17H12

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human IL-2, PE-Cy7 (Clone: MQ1-17H12) 检测试剂 - 结果示例图片Anti-Human IL-2, PE-Cy7 (Clone: MQ1-17H12) 检测试剂 - 结果示例图片
结果示例

图 - 正常人的外周血样本用细胞刺激阻断剂(目录号:CS1001和CS1002)预处理,固定破膜剂(目录号:GAS003/2)破膜后进行染色,左图为Rat IgG2a, κ Isotype control, PE-Cy7和Anti-Human CD3,APC共染的结果,右图为Anti-Human IL-2, PE-Cy7和Anti-Human CD3, APC共染的结果。以淋巴细胞设门进行分析。

分子信息

概述人 IL2, IL-2 靶点信息

IL2 分子靶点信息概述

  • 分子名:IL2, interleukin 2
  • 基因家族:Interleukins
  • 别名:IL-2; TCGF
  • 全称:T cell growth factor

IL2 分子靶点综述

白细胞介素2(IL-2)是15kDa的糖蛋白,在人类中,由位于4号染色体q26–28区域的单基因编码。它是免疫系统中的一类信号分子,调控对具有免疫功能的白血球(白细胞,通常是淋巴细胞)的活性。IL-2是机体对微生物感染的天然应答,区别外来和自身抗原。IL-2通过与IL-2受体来发挥功能,其受体由3条链组成,分别为α链(CD25)、β链(CD122)和γ链(CD132)。监测血清中的IL-2含量为多种病理状态提供更详细的视角,包括癌症、传染病、移植排斥、多发性硬化症、类风湿性关节炎、系统性红斑狼疮和I型糖尿病等。

人 Human IL2 分子靶点信息

  • 分子名:IL2, interleukin 2
  • 别称:
    • aldesleukin
    • IL-2
    • interleukin-2
    • involved in regulation of T-cell clonal expansion
    • lymphokine
    • T cell growth factor
    • TCGF
  • 基因序列:NCBI_Gene: 3558
  • 蛋白序列:UniProtKB: P60568

人 Human IL2靶点分子功能(预测)

Enables cytokine activity and interleukin-2 receptor binding activity. Involved in leukocyte activation involved in immune response; positive regulation of interleukin-17 production; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within negative regulation of B cell apoptotic process; positive regulation of B cell proliferation; and positive regulation of activated T cell proliferation. Predicted to be located in extracellular space. Implicated in several diseases, including Takayasu's arteritis; auditory system disease (multiple); autoimmune disease (multiple); carcinoma (multiple); and neurodegenerative disease (multiple). Biomarker of several diseases, including Zellweger syndrome; aggressive periodontitis; anogenital venereal wart; autoimmune disease (multiple); and uveitis (multiple).

操作步骤


下载本产品说明书

使用方法

请注意该指标为胞内因子,染色前需进行固定破膜操作。

全血样本:取100μL全血样本,经裂红、固定破膜处理后,加入5μL Anti-Human IL-2,PE-Cy7和其他胞内抗体,震荡混匀,于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入1-2mL 流式染色缓冲液(目录号:S1001),300×g 离心 5 - 10分钟,弃上清,加入500μL 流式染色缓冲液重悬,上机检测。

其他样本:将 1×105 - 108 个单细胞经裂红(若需)、固定破膜处理后,取100uL,加入5μL AntiHuman IL-2, PE-Cy7和其他胞内抗体,使反应终体积为 100μL 。震荡混匀,于室温避光孵育15分钟或 2 - 8℃避光孵育30分钟。每管加入 1 - 2mL 流式染色缓冲液,300 × g离心 5 - 10分钟,弃上清,加入 500μL 流式染色缓冲液重悬,上机检测。

实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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