EK218

Mouse IL-18 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：IL18, IGIF, IL1F4
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
灵敏度：0.93 pg/mL
检测范围：62.5 pg/mL - 4000 pg/mL
回收率：80% - 119%

在售SKU:70-EK218-48, 70-EK218-96

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库存号

描述

文章目录[隐藏]

分子信息
IL18 分子靶点信息概述
IL18 分子靶点综述
- 小鼠 Mouse Il18 分子靶点信息
- 小鼠 Mouse Il18 靶点分子功能（预测）

商品名	Mouse IL-18 ELISA Kit (小鼠白细胞介素18 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.7% - 4.0%；板间变异系数：2.4% - 3.7%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	血清血浆：20 μL；细胞培养上清：100 μL
灵敏度	0.93 pg/mL
检测范围	62.5 pg/mL - 4000 pg/mL
回收率	80% - 119%
平均回收率	0.98
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗小鼠IL-18单克隆抗体的96孔聚苯乙烯酶标板小鼠IL-18 冻干标准品 IL-18 检测抗体辣根过氧化物酶标记的链霉亲和素检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-18抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的IL-18与固相抗体结合和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-18的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-18 ELISA Kit (小鼠白细胞介素18 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 IL18, IGIF, IL1F4 靶点信息

IL18 分子靶点信息概述

分子名：IL18, interleukin 18
基因家族：Interleukins
别名：IGIF; IL1F4; IL-1g; IL-18
全称：interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18 分子靶点综述

白细胞介素18(IL-18)，又名干扰素γ诱导因子，是一种促炎性细胞因子，在人类中由IL18基因编码。IL-18属于IL-1超家族，由巨噬细胞和其它细胞分泌产生。IL-18刺激后，自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素，进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应，同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。

小鼠 Mouse Il18 分子靶点信息

分子名：Il18, interleukin 18
别称：
- Igif
- Il-18
- interferon gamma inducing factor
基因序列：NCBI_Gene: 16173
蛋白序列：

小鼠 Mouse Il18 靶点分子功能（预测）

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).