EK109

Human IL-9 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:IL9, IL-9, P40, HP40
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:0.06 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:80% - 117%

在售SKU:70-EK109-48, 70-EK109-96, 人白细胞介素9, 白细胞介素9, 白细胞介素, 人, 白介素, 9

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描述

商品名

Human IL-9 ELISA Kit (人白细胞介素9ELISA 试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.9% - 3.1%;板间变异系数:3.3% - 5.7%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

0.06 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

80% - 117%

平均回收率

1.04

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-9单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-9冻干标准品
  • IL-9 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-9与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-9的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-9 ELISA Kit (人白细胞介素9ELISA 试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL9, IL-9, P40, HP40 靶点信息

IL9 分子靶点信息概述

  • 分子名:IL9, interleukin 9
  • 基因家族:Interleukins
  • 别名:IL-9; HP40; P40
  • 全称:p40 T-cell and mast cell growth factor; T-cell growth factor p40; p40 cytokine; homolog of mouse T cell and mast cell growth factor 40

IL9 分子靶点综述

白细胞介素9(IL-9)是一个分子量为14kDa的糖基化蛋白,包含10个参与二硫键连接的半胱氨酸残基。它是由T细胞特别是CD4+辅助性细胞产生的细胞因子,可调节多种造血细胞。该细胞因子刺激细胞增殖、阻止细胞凋亡。它是通过与IL-9受体结合起作用的,可激活不同的信号转导及激活(STAT)蛋白,从而参与多种生物过程。编码该细胞因子的基因已被确定为哮喘的候选基因。小鼠模型的哮喘遗传研究表明,这种细胞因子是支气管高反应发病机制的一个决定性因素。IL-9介导的自分泌环的存在暗示着一些恶性肿瘤如霍奇金病的存在。IL-9由李-斯二氏细胞、霍奇金淋巴瘤细胞和一些大型再生障碍性淋巴瘤细胞表达,而非霍奇金淋巴瘤和外周T细胞淋巴瘤不表达。

人 Human IL9 分子靶点信息

  • 分子名:IL9, interleukin 9
  • 别称:
    • cytokine P40
    • homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL-9
    • interleukin-9
    • P40
    • p40 cytokine
    • p40 T-cell and mast cell growth factor
    • T-cell growth factor p40
  • 基因序列:NCBI_Gene: 3578
  • 蛋白序列:UniProtKB: P15248

人 Human IL9靶点分子功能(预测)

Predicted to enable cytokine activity and interleukin-9 receptor binding activity. Predicted to be involved in positive regulation of interleukin-5 production. Predicted to act upstream of or within several processes, including B cell activation; regulation of cellular protein metabolic process; and regulation of receptor signaling pathway via JAK-STAT. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in asthma and respiratory syncytial virus infectious disease. Biomarker of COVID-19; asthma; cystic fibrosis; lung disease (multiple); and rhinitis.

操作步骤


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引用文献


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