F11003C05

Anti-Human CD3, PE-Cy7 (Clone:HIT3a) 检测试剂

¥1,304.00 – ¥2,664.00

说明书

分子靶点：CD3
种属：人 (Human)
标记：PE-Cy7
亚型：Mouse IgG1, κ
克隆号：HIT3a

在售SKU:70-F11003C05-25, 70-F11003C05-100, 人, CD3, CD3, CD3, CD3, CD3

¥<\/span>2,664.00<\/bdi><\/span><\/span>","sku":"70-F11003C05-100","variation_description":"","variation_id":18020,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"25T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":1304,"display_regular_price":1304,"image":{"title":"1656571413-fc-antibody.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/1656571413-fc-antibody.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":16568,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>1,304.00<\/bdi><\/span><\/span>","sku":"70-F11003C05-25","variation_description":"","variation_id":18021,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

结果示例

商品名	Anti-Human CD3, PE-Cy7 (Clone:HIT3a) 检测试剂
靶点
标记	PE-Cy7
仪器	适用488nm, 532nm, 561nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	HIT3a
推荐应用	流式检测
用量	5 μL/Test
纯化	亲和层析
储存液	含0.2% BSA和0.02% ProClin950的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色Anti-Human CD3, PE-Cy7 (F11003C05) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取100 μl抗凝血，加入5 μl Anti-Human CD3, PE-Cy7和其他抗体，震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g离心5 - 10分钟，弃上清，加入500 μl流式染色缓冲液重悬，上机检测。
其他样本：将1×10⁵ - 10⁸个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human CD3, PE-Cy7和其他抗体，使反应终体积为100 μl。震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入1 - 2 ml流式染色缓冲液，300 × g离心5 - 10分钟，弃上清，加入500 μl流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。