F11003C05

Anti-Human CD3, PE-Cy7 (Clone:HIT3a) 检测试剂

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¥1,304.00¥2,664.00

  • 分子靶点:CD3
  • 种属:人 (Human)
  • 标记:PE-Cy7
  • 亚型:Mouse IgG1, κ
  • 克隆号:HIT3a

在售SKU:70-F11003C05-25, 70-F11003C05-100, 人, CD3, CD3, CD3, CD3, CD3

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描述

文章目录[隐藏]

商品名

Anti-Human CD3, PE-Cy7 (Clone:HIT3a) 检测试剂

靶点

标记

PE-Cy7

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

HIT3a

推荐应用

流式检测

用量

5 μL/Test

纯化

亲和层析

储存液

含0.2% BSA和0.02% ProClin950的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD3, PE-Cy7 (Clone:HIT3a) 检测试剂 - 结果示例图片
结果示例

图 - 人外周血染色Anti-Human CD3, PE-Cy7 (F11003C05) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

操作步骤


下载本产品说明书

使用方法

全血样本:取100 μl抗凝血,加入5 μl Anti-Human CD3, PE-Cy7和其他抗体,震荡混匀,于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300 × g离心5 - 10分钟,弃上清,加入500 μl流式染色缓冲液重悬,上机检测。
其他样本:将1×105 - 108个单细胞经适当离心后,取适量流式染色缓冲液 (目录号S1001)重悬,加入5 μl Anti-Human CD3, PE-Cy7和其他抗体,使反应终体积为100 μl。震荡混匀,于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入1 - 2 ml流式染色缓冲液,300 × g离心5 - 10分钟,弃上清,加入500 μl流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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