F110TA03

Anti-Human TNF-α, APC (Clone: MAb11) 检测试剂

¥790.00 – ¥3,180.00

说明书

分子靶点：TNF, TNFA, TNFSF2
种属：人 (Human)
标记：APC
亚型：Mouse IgG1, κ
克隆号：MAb11

在售SKU,F110TA03, 70-F110TA03-25, 70-F110TA03-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 人 Human TNF 分子靶点信息
- 人 Human TNF靶点分子功能（预测）

商品名	Anti-Human TNF-α, APC (Clone: MAb11) 检测试剂
靶点	TNF, TNFA, TNFSF2
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	MAb11
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 正常人的外周血样本用细胞刺激阻断剂（目录号：CS1001和CS1002）预处理，固定破膜剂（目录号：GAS003/2）破膜后进行染色，左图为Mouse IgG1, κ Isotype control, APC和Anti-Human CD3,FITC共染的结果，右图为Anti-Human TNF-α, APC和Anti-Human CD3, FITC共染的结果。以淋巴细胞设门进行分析。

分子信息

概述人 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

人 Human TNF 分子靶点信息

分子名：TNF, tumor necrosis factor
别称：
- APC1 protein
- cachectin
- DIF
- TNF superfamily, member 2
- TNF-a
- TNF-alpha
- TNF, macrophage-derived
- TNF, monocyte-derived
- TNFA
- TNFSF2
- TNLG1F
- tumor necrosis factor alpha
- tumor necrosis factor ligand 1F
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor-alpha
基因序列：NCBI_Gene: 7124
蛋白序列：UniProtKB: P01375

人 Human TNF靶点分子功能（预测）

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

请注意该指标为胞内因子，染色前需进行固定破膜操作。

全血样本：取100μL全血样本，经裂红、固定破膜处理后，加入5μL Anti-Human TNF-α, APC 震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入 1-2mL 流式染色缓冲液（目录号：S1001），300×g 离心 5 - 10分钟，弃上清，加入 500μL 流式染色缓冲液重悬，上机检测。

其他样本：将 1×10⁵ - 10⁸个单细胞经裂红（若需）、固定破膜处理后，取100uL，加入5μL AntiHuman TNF-α, APC，使反应终体积为 100μL 。震荡混匀，于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入 1 - 2mL 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500μL 流式染色缓冲液重悬，上机检测。

实验中，可对抗体进行优化滴定以获得最佳效果。