F110TA05

Anti-Human TNF-α, PE-Cy7 (Clone: MAb11) 检测试剂

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¥1,470.00¥3,385.00

  • 分子靶点:TNF, TNFA, TNFSF2
  • 种属:人 (Human)
  • 标记:PE-Cy7
  • 亚型:Mouse IgG1, κ
  • 克隆号:MAb11

在售SKU,F110TA05, 70-F110TA05-25, 70-F110TA05-100

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描述

商品名

Anti-Human TNF-α, PE-Cy7 (Clone: MAb11) 检测试剂

靶点

TNF, TNFA, TNFSF2

标记

PE-Cy7

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

MAb11

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human TNF-α, PE-Cy7 (Clone: MAb11) 检测试剂 - 结果示例图片Anti-Human TNF-α, PE-Cy7 (Clone: MAb11) 检测试剂 - 结果示例图片
结果示例

图 - 正常人的外周血样本用细胞刺激阻断剂(目录号:CS1001和CS1002)预处理,固定破膜剂(目录号:GAS003/2)破膜后进行染色,左图为Mouse IgG1, κ Isotype control, PE-Cy7和Anti-Human CD3,APC共染的结果,右图为Anti-Human TNF-α, PE-Cy7和Anti-Human CD3, APC共染的结果。以淋巴设门进行分析。

分子信息

概述人 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

人 Human TNF 分子靶点信息

  • 分子名:TNF, tumor necrosis factor
  • 别称:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • 基因序列:NCBI_Gene: 7124
  • 蛋白序列:UniProtKB: P01375

人 Human TNF靶点分子功能(预测)

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

操作步骤


下载本产品说明书

使用方法

请注意该指标为胞内因子,染色前需进行固定破膜操作。

全血样本:取100μL全血样本,经裂红、固定破膜处理后,加入5μL Anti-Human TNF-α, PE-Cy7 震荡混匀,于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入 1-2mL 流式染色缓冲液(目录号:S1001),300×g 离心 5 - 10分钟,弃上清,加入 500μL 流式染色缓冲液重悬,上机检测。

其他样本:将 1×105 - 108个单细胞经裂红(若需)、固定破膜处理后,取100uL,加入5μL AntiHuman TNF-α, PE-Cy7,使反应终体积为 100μL 。震荡混匀,于室温避光孵育15分钟或2 - 8℃避光孵育30分钟。每管加入 1 - 2mL 流式染色缓冲液,300 × g 离心 5 - 10分钟,弃上清,加入 500μL 流式染色缓冲液重悬,上机检测。

实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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