EK218EG

EasyGo!™ Mouse IL-18 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

X

¥2,190.00¥3,650.00

  • 分子靶点:IL18, IGIF, IL1F4
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,DMEM 细胞培养上清
  • 检测样本体积:20 μL
  • 灵敏度:0.35 pg/mL
  • 检测范围:3.125 pg/mL - 200 pg/mL
  • 回收率:88% - 114%

在售SKU: 70-EK218EG-48, 70-EK218EG-96, EK218EG, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

¥<\/span>2,190.00<\/bdi><\/span><\/span>","sku":"70-EK218EG-48","variation_description":"","variation_id":19220,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":3650,"display_regular_price":3650,"image":{"title":"EasyGo-1","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/EasyGo-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":18334,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>3,650.00<\/bdi><\/span><\/span>","sku":"70-EK218EG-96","variation_description":"","variation_id":19221,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

描述

商品名

EasyGo!™ Mouse IL-18 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

一步法

精密度

板内变异系数:3.4% - 4.6%;板间变异系数:3.1% - 5.4%

样本类型

血清,血浆,DMEM 细胞培养上清

检测样本体积

20 μL

灵敏度

0.35 pg/mL

检测范围

3.125 pg/mL - 200 pg/mL

回收率

88% - 114%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,2-8℃保存。已拆开,标准品-20℃保存,其它2-8℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™ Mouse IL-18 One-Step ELISA Kit EasyGo!™ 小鼠白介素 18 (IL-18) 一步法酶联免疫吸附测定试剂盒 - 标准曲线
标准曲线

分子信息

概述小鼠 IL18, IGIF, IL1F4 靶点信息

IL18 分子靶点信息概述

  • 分子名:IL18, interleukin 18
  • 基因家族:Interleukins
  • 别名:IGIF; IL1F4; IL-1g; IL-18
  • 全称:interferon-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)

IL18 分子靶点综述

白细胞介素18(IL-18),又名干扰素γ诱导因子,是一种促炎性细胞因子,在人类中由IL18基因编码。IL-18属于IL-1超家族,由巨噬细胞和其它细胞分泌产生。IL-18刺激后,自然杀伤细胞(NK)和某些T细胞就会释放另一种重要的细胞因子—干扰素γ或Ⅱ型干扰素,进而激活巨噬细胞或其它细胞。IL-18可诱导产生严重的炎症反应,同样可增加人类神经元中β-淀粉样蛋白(与阿尔茨海默氏病相关)的产生。

小鼠 Mouse Il18 分子靶点信息

小鼠 Mouse Il18 靶点分子功能(预测)

Enables cytokine activity. Involved in lipid homeostasis; positive regulation of cell differentiation; and positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including interleukin-18-mediated signaling pathway; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interferon-gamma production. Located in extracellular space. Is expressed in several structures, including back skin; dorsal aorta; gut; lymphatic system; and reproductive system. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; allergic rhinitis; autoimmune disease (multiple); biliary atresia; and hepatitis B. Orthologous to human IL18 (interleukin 18).

操作步骤

检验步骤概要

  1. 1) 标准品孔加入 100 μL 2 倍倍比稀释的标准品。空白孔加入 100 μL 1×检测缓冲液或培养基。
  2. 血清/血浆:样本孔加入 80 μL 标准品稀释液和 20 μL 样本。细胞培养上清:样本孔加入 100 μL 细胞培养上清。
  3. 37℃孵育 30 min,或室温(25℃±3℃)孵育 1 小时。撕膜,洗涤 3 次。
  4. 每孔加入 100 μL 显色底物,避光,室温(25℃±3℃)孵育 10±5 分钟。
  5. 每孔加入 100 μL 终止液。
  6. 30 分钟内,在 450nm 波长检测 OD 值,参考波长 570nm 或 630nm。

检测步骤简略图示

下载本产品说明书

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


评价

目前还没有评价

成为第一个“EasyGo!™ Mouse IL-18 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注