EK282S

Mouse TNF-α Standard (小鼠TNF-α 标准品)

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¥180.00

  • 分子靶点：TNF, TNFA, TNFSF2
  • 种属：小鼠 (Mouse)
  • 试剂盒：EK282
  • 保存：短期4℃保存，长期-20℃保存
  • 运输条件：4℃蓝冰运输

在售SKU:70-EK282S

描述

本产品只包含标准品试剂，如需购买试剂盒请点击下图

商品名 Mouse TNF-α Standard (小鼠TNF-α 标准品)
组分 小鼠TNF-α标准品
检测方法 双抗夹心法
样本类型 血清，血浆，细胞培养上清及其他生物学样本
板式 管
保存 短期4℃，长期-20℃保存
运输条件 4℃蓝冰运输

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse TNF-α ELISA Kit (小鼠TNF-α ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名：TNF, tumor necrosis factor
  • 基因家族：Tumor necrosis factor superfamily
  • 别名：TNFSF2; DIF; TNF-alpha
  • 曾用名：TNFA
  • 全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

小鼠 Mouse Tnf 分子靶点信息

小鼠 Mouse Tnf 靶点分子功能（预测）

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

检测步骤


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ELISA技术在细胞治疗中的应用ELISA技术在细胞治疗中的应用 

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血清OR血浆，哪个是ELISA的菜 

ELISA系列 | 血管内皮生长因子（VEGF） 

ELISA通关必备丨数据篇丨标准曲线不佳 

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ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

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