EK382

Rat TNF-a ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥10,800.00

分子靶点：TNF, TNFA, TNFSF2
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：10 μL
灵敏度：0.43 pg/mL
检测范围：4.69 pg/mL - 300 pg/mL
回收率：82% - 116%

在售SKU:70-EK382/3-48, 70-EK382/3-96

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Size
库存号

描述

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 大鼠 Rat Tnf 分子靶点信息
- 大鼠 Rat Tnf 靶点分子功能（预测）

商品名	Rat TNF-α ELISA Kit (大鼠肿瘤坏死因子α ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.4% - 6.3%；板间变异系数：6.1% - 8.4%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	10 μL
灵敏度	0.43 pg/mL
检测范围	4.69 pg/mL - 300 pg/mL
回收率	82% - 116%
平均回收率	0.96
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TNF-α单克隆抗体的96孔聚苯乙烯酶标板大鼠TNF-α冻干标准品 TNF-α检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat TNF-α ELISA Kit (大鼠肿瘤坏死因子α ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

大鼠 Rat Tnf 分子靶点信息

分子名：Tnf, tumor necrosis factor
别称：
- cachectin
- LOC103694380
- MGC124630
- RATTNF
- TNF-a
- TNF-alpha
- Tnfa
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor superfamily member 2
- tumor necrosis factor superfamily, member 2
- tumor necrosis factor-alpha
- tumor necrosis factor-like
基因序列：NCBI_Gene: 24835
蛋白序列：UniProtKB: P16599

大鼠 Rat Tnf 靶点分子功能（预测）

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).