EK106EGA

EasyGo!™ Human IL-6 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,190.00¥3,650.00

  • 分子靶点:IL6, IL-6, IFNB2, HGF
  • 种属:人 (Human)
  • 样本类型:血清,血浆
  • 检测样本体积:20 μL
  • 灵敏度:1.05 pg/mL
  • 检测范围:1.56 pg/mL - 100 pg/mL
  • 回收率:76% - 125%

在售SKU:70-EK106EGA-48, 70-EK106EGA-96, 人白细胞介素6, 白细胞介素6, 白细胞介素, 人, 白介素, 6, 一步法, 一步法ELISA, 一步法试剂盒, 一步法ELISA试剂盒

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描述

商品名

EasyGo!™ Human IL-6 One-Step ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

一步法

精密度

板内变异系数:7.1% - 9.5%;板间变异系数:2.8% - 9.2%

样本类型

血清,血浆

检测样本体积

20 μL

灵敏度

1.05 pg/mL

检测范围

1.56 pg/mL - 100 pg/mL

回收率

76% - 125%

平均回收率

1.00

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 含微球的预包被酶标板
  • 标准品
  • 标准品稀释液
  • 显色底物
  • 终止液
  • 20×洗液

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上,酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后,加入显色底物(TMB),避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应,在 450nm 波长(参考波长 570 - 630 nm)测定吸光度值。

EasyGo!™ Human IL-6 One-Step ELISA Kit EasyGo!™ 人白细胞介素6 (IL-6) 一步法酶联免疫吸附测定试剂盒- 标准曲线
标准曲线

分子信息

概述人 IL6, IL-6, IFNB2, HGF 靶点信息

IL6 分子靶点信息概述

  • 分子名:IL6, interleukin 6
  • 基因家族:Interferons; Interleukins; Interleukin 6 type cytokine family
  • 别名:IL-6; BSF2; HGF; HSF
  • 曾用名:IFNB2
  • 全称:interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶点综述

白细胞介素6(IL-6)是一种同时具备促炎性和抗炎性的细胞因子。在人类中,它由IL-6基因编码。在感染期间和受到外伤后,尤其是烧伤或其它组织损伤导致的炎症,IL-6会由T细胞和巨噬细胞分泌,促进免疫应答。研究表明,小鼠抵抗肺炎链球菌感染时需要IL-6的参与,说明IL-6在抵抗感染中发挥作用。IL-6在很多疾病如糖尿病、动脉粥样硬化、抑郁症、阿尔茨海默氏病、系统性红斑狼疮、多发性骨髓瘤、前列腺癌、白塞氏病和类风湿性关节炎等中可促进炎性和自身免疫疾病进程。晚期/转移性癌症患者的血液中具有较高水平的IL-6。

人 Human IL6 分子靶点信息

  • 分子名:IL6, interleukin 6
  • 别称:
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • 基因序列:NCBI_Gene: 3569
  • 蛋白序列:UniProtKB: P05231

人 Human IL6靶点分子功能(预测)

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

操作步骤

检验步骤概要

  1. 1)标准品孔加入100 μL2倍倍比稀释的标准品。空白孔加入100 μL标准品稀释液或培养基。
  2. 2)血清/血浆:样本孔加入80 μL 1×PBST(即1×洗液)和20 μL样本。细胞培养上清:样本孔加入100 μL细胞培养上清。
  3. 37℃孵育 1 小时,或室温(25℃±3℃)孵育 2小时。撕膜,洗涤 3 次。
  4. 每孔加入 100 μL 显色底物,避光,室温(25℃±3℃)孵育 10±5 分钟。
  5. 每孔加入 100 μL 终止液。
  6. 30 分钟内,在 450nm 波长检测 OD 值,参考波长 570nm 或 630nm。

检测步骤简略图示

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