F11045A03

Anti-Human CD45, APC (Clone:HI30)流式抗体 (新品)

¥440.00 – ¥968.00

说明书

分子靶点：CD45, GP180, PTPRC
种属：人 (Human)
标记：APC
亚型：Mouse IgG1, κ
克隆号：HI30

在售SKU, 70-AH04505-20, 70-AH04505-50, 70-AH04505-100, AH04505, 70-F11045A03-25, 70-F11045A03-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
PTPRC 分子靶点信息概述
PTPRC 分子靶点综述
- 人 Human PTPRC 分子靶点信息
- 人 Human PTPRC靶点分子功能（预测）

商品名	Anti-Human CD45, APC (Clone:HI30)流式抗体
靶点	CD45, GP180, PTPRC
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	HI30
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD45, APC (F11045A03)的结果。

分子信息

概述人 CD45, GP180, PTPRC 靶点信息

PTPRC 分子靶点信息概述

分子名：PTPRC, protein tyrosine phosphatase receptor type C
基因家族：CD molecules; Fibronectin type III domain containing; Protein tyrosine phosphatases receptor type
别名：LCA; T200; GP180
曾用名：CD45

PTPRC 分子靶点综述

CD45也被称为白细胞共同抗原(leukocyte common antigen, LCA)，由一类结构相似，分子量较大(180 kDa、190 kDa、205 kDa和200 kDa)的跨膜蛋白组成，广泛存在于所有白细胞表面(包括淋巴细胞、粒细胞和单核细胞)，但不表达于成熟的红细胞、血小板、骨髓中的成熟红系细胞以及非造血组织。CD45胞浆区段具有蛋白质酪氨酸磷酸酶的作用，能使底物P56lck和P59fyn上酪氨酸脱磷酸而激活，在细胞的信息传导中发挥重要作用，CD45是细胞膜上信号传导的关键分子，在淋巴细胞的发育成熟，功能调节及信号传递中具有重要意义。CD45的分布可作为某些T细胞亚群的分类标志。

人 Human PTPRC 分子靶点信息

分子名：PTPRC, protein tyrosine phosphatase receptor type C
别称：
- B220
- CD45
- CD45 antigen
- CD45R
- glycoprotein
- GP180
- L-CA
- LCA
- leukocyte common antigen
- leukocyte-common antigen
- LY5
- protein tyrosine phosphatase, receptor type, c polypeptide
- receptor-type tyrosine-protein phosphatase C
- T200
- T200 glycoprotein
- T200 leukocyte common antigen
基因序列：NCBI_Gene: 5788
蛋白序列：UniProtKB: P08575

人 Human PTPRC靶点分子功能（预测）

Enables cytoskeletal protein binding activity; protein kinase binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including hematopoietic or lymphoid organ development; positive regulation of cell population proliferation; and regulation of protein kinase activity. Located in bleb and external side of plasma membrane. Implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive.

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD45, APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300× g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD45, APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。