F11056A02

Anti-Human CD56 (NCAM), PE (Clone:MY31)流式抗体 (新品)

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¥600.00¥1,320.00

  • 分子靶点:CD56, NCAM1, NCAM
  • 种属:人 (Human)
  • 标记:PE
  • 亚型:Mouse IgG1, κ
  • 克隆号:MY31

在售SKU, 70-AH05604-20, 70-AH05604-50, 70-AH05604-100, AH05604, 70-F11056A02-25, 70-F11056A02-100

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描述

商品名

Anti-Human CD56 (NCAM), PE (Clone:MY31)流式抗体

靶点

CD56, NCAM1, NCAM

标记

PE

仪器

适用488nm, 532nm, 561nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

MY31

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD56 (NCAM), PE (Clone:MY31)流式抗体 - 结果示例图片
结果示例

图 - 人外周血染色Anti-Human CD56, PE (F11056A02) 和 Anti-Human CD3, APC (F1100303) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD56, NCAM1, NCAM 靶点信息

NCAM1 分子靶点信息概述

  • 分子名:NCAM1, neural cell adhesion molecule 1
  • 基因家族:CD molecules; Fibronectin type III domain containing; I-set domain containing; Ig-like cell adhesion molecule family
  • 别名:NCAM; CD56

NCAM1 分子靶点综述

CD56也称为神经细胞黏着分子(Neural Cell Adhesion molecule, NCAM)、Leu-19或NKH1,是分子量为140 kD的跨膜糖蛋白。CD56表达于神经元,在神经细胞的同质粘附中发挥作用。在造血系统中,它表达于NK和NKT细胞,在细胞粘附中发挥作用。

人 Human NCAM1 分子靶点信息

  • 分子名:NCAM1, neural cell adhesion molecule 1
  • 别称:
    • antigen recognized by monoclonal antibody 5.1H11
    • CD56
    • MSK39
    • N-CAM-1
    • NCAM
    • NCAM-1
    • neural cell adhesion molecule, NCAM
  • 基因序列:NCBI_Gene: 4684
  • 蛋白序列:UniProtKB: P13591

人 Human NCAM1靶点分子功能(预测)

Predicted to enable virus receptor activity. Predicted to be involved in commissural neuron axon guidance; modulation of chemical synaptic transmission; and regulation of semaphorin-plexin signaling pathway. Predicted to act upstream of or within several processes, including homotypic cell-cell adhesion; positive regulation of calcium-mediated signaling; and regulation of exocyst assembly. Located in cytosol and external side of plasma membrane. Implicated in bipolar disorder; middle cerebral artery infarction; and pancreatic cancer. Biomarker of several diseases, including acute promyelocytic leukemia; brain glioblastoma multiforme; cholangiocarcinoma; insulinoma; and ovarian serous carcinoma.

操作步骤


下载本产品说明书

使用方法

全血样本:取 100 μl 抗凝血,加入 5 μl Anti-Human CD56, PE 和其他抗体,震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300× g离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号 S1001)重悬,加入 5 μl Anti-Human CD56, PE 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

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