F1101409

Anti-Human CD14, mFluor 610 (Clone: 61D3)检测试剂

¥1,288.00 – ¥2,576.00

说明书

分子靶点：CD14
种属：人 (Human)
标记：mFlour 610
亚型：Mouse IgG1, κ
克隆号：61D3

在售SKU, F1101409, 70-F1101409-25, 70-F1101409-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD14 分子靶点信息概述
CD14 分子靶点综述
- 人 Human CD14 分子靶点信息
- 人 Human CD14靶点分子功能（预测）

商品名	Anti-Human CD14, mFluor 610 (Clone: 61D3) 检测试剂
靶点	CD14
标记	mFlour 610
仪器	适用405nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	61D3
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血进行染色，绿色线为Mouse IgG1, κ Isotype control, mFluor 610的结果，红色线为Anti-Human CD14, mFluor 610的结果。以单核细胞设门进行分析。

分子信息

概述人 CD14 靶点信息

CD14 分子靶点信息概述

分子名：CD14, CD14 molecule
基因家族：CD molecules; Scavenger receptors
全称：CD14 antigen

CD14 分子靶点综述

CD14是一种糖磷脂酰肌醇相关的膜蛋白，参与内毒素结合和识别凋亡细胞。CD14抗体可用于研究单核细胞，树突状细胞，组织细胞，肝脏窦状小管中的枯否细胞等。CD14与S100，CD68用于研究组织细胞增多的淋巴结增生性疾病。在单核细胞白血病和组织细胞增生淋巴瘤中肿瘤细胞呈CD14阳性。CD14抗体可以作为弥漫性大B细胞淋巴瘤以及脾边缘区淋巴瘤的参考依据，其它B细胞淋巴瘤除外。

人 Human CD14 分子靶点信息

分子名：CD14, CD14 molecule
别称：
- cd14 antigen
- monocyte differentiation antigen CD14
- myeloid cell-specific leucine-rich glycoprotein
基因序列：NCBI_Gene: 929
蛋白序列：UniProtKB: P08571

人 Human CD14靶点分子功能（预测）

Enables lipopeptide binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Involved in cellular response to bacterial lipopeptide; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interleukin-8 production. Acts upstream of or within cellular response to lipopolysaccharide; cellular response to lipoteichoic acid; and positive regulation of tumor necrosis factor production. Located in Golgi apparatus and membrane raft. Is anchored component of external side of plasma membrane. Part of lipopolysaccharide receptor complex. Implicated in several diseases, including IgA glomerulonephritis; artery disease (multiple); celiac disease; cystic fibrosis; and pulmonary tuberculosis. Biomarker of several diseases, including biliary atresia; cystic fibrosis; lung disease (multiple); perinatal necrotizing enterocolitis; and polycystic kidney disease (multiple).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取100 μl 抗凝血，加入5 μl Anti-Human CD14, mFluor 610和其他抗体，震荡混匀，于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl
Anti-Human CD14, mFluor 610和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光
孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 -
10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。