F11011b03

Anti-Human CD11b, APC （Clone: LT11）流式抗体 (新品)

¥680.00 – ¥1,504.00

说明书

分子靶点：CD11B, MAC-1, ITGAM, CR3A
种属：人 (Human)
标记：APC
亚型：Mouse IgG1
克隆号：LT11

在售SKU, 70-AH011B05-20, 70-AH011B05-50, 70-AH011B05-100, AH011B05, 70-F11011B03-25, 70-F11011B03-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
ITGAM 分子靶点信息概述
ITGAM 分子靶点综述

商品名	Anti-Human CD11b, APC （Clone: LT11）流式抗体
靶点	CD11B, MAC-1, ITGAM, CR3A
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Mouse IgG1
克隆号	LT11
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色左图为Mouse IgG1 Istype control,APC，右图为 Anti-Human CD11b, APC（Clone: LT11）(F11011b03/3) 的结果。以淋巴细胞、粒细胞和单核细胞设门进行分析。

分子信息

概述

ITGAM 分子靶点信息概述

分子名：ITGAM, integrin subunit alpha M
基因家族：CD molecules; Integrin alpha subunits; Complement system regulators and receptors
别名：MAC-1; CD11b
曾用名：CR3A; CD11B
全称：complement component 3 receptor 3 subunit; macrophage-1 antigen alpha subunit; integrin, alpha M (complement component receptor 3, alpha; also known as CD11b (p170), macrophage antigen alpha polypeptide); integrin, alpha M (complement component 3 receptor 3 subunit)

ITGAM 分子靶点综述

CD11b与CD18非共价结合，形成αM-β2整合素(Mac-1)，与CD54(ICAM-1)、C3bi和纤维蛋白原结合。Mac-1表达于巨噬细胞、NK细胞、粒细胞、小胶质细胞、活化的淋巴细胞和小鼠B-1细胞。CD11b与其配体结合，参与粘附细胞的互相作用。此抗体也可交叉识别人CD11b，可检测人外周血中单核细胞、粒细胞和某一群NK细胞上的该抗原。

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取100 μl 抗凝血，加入5 μl Anti-Human CD11b,APC 和其他抗体，震荡混匀，于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human CD11b,APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。