F2120603

Anti-Mouse CD206, APC (Clone: C068C2) 检测试剂

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¥580.00¥1,280.00

  • 分子靶点:MRC1, CD206
  • 种属:小鼠 (Mouse)
  • 标记:APC
  • 亚型:Mouse IgG2a, κ
  • 克隆号:C068C2

在售SKU,F2120603, 70-F2120603-25, 70-F2120603-100, CD206

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描述

商品名

Anti-Mouse CD206, APC (Clone: C068C2) 检测试剂

靶点

MRC1, CD206

标记

APC

仪器

适用633nm激光器的流式细胞仪

亚型

Mouse IgG2a, κ

克隆号

C068C2

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含0.2% BSA和 0.2%ProClin950的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Mouse CD206, APC (Clone: C068C2) 检测试剂 - 结果示例图片Anti-Mouse CD206, APC (Clone: C068C2) 检测试剂 - 结果示例图片
结果示例

图 - 以 FSC/SSC 中的所有细胞设门进行分析的小鼠腹腔巨噬细胞本产品的结果示例,左图为Anti-Mouse F4/80, PE-Cy7和Rat IgG2a,κ, APC染色结果,右图为Anti-Mouse F4/80, PE-Cy7Anti-Mouse CD206, APC染色结果。

分子信息

概述小鼠 MRC1, CD206 靶点信息

MRC1 分子靶点信息概述

  • 分子名:MRC1, mannose receptor C-type 1
  • 基因家族:Scavenger receptors; C-type lectin domain containing; CD molecules; MicroRNA protein coding host genes
  • 别名:CLEC13D; CD206; bA541I19.1; CLEC13DL
  • 曾用名:MRC1L1
  • 全称:mannose receptor, C type 1-like 1; mannose receptor, C type 1; macrophage mannose receptor

MRC1 分子靶点综述

CD206又名MSR(甘露糖受体),是一个175kDa的跨膜蛋白。CD206主要表达在树突状细胞和巨噬细胞中,在先天和自适应免疫中发挥作用。CD206也在脑组织中的小胶质细胞和mato细胞中表达。CD206主要参与细胞粘附和宿主防御机制,也有报到CD206和阿尔兹海默症中动脉粥样硬化和淀粉样β沉积发展有关。

小鼠 Mouse Mrc1 分子靶点信息

小鼠 Mouse Cd55 靶点分子功能(预测)

Enables mannose binding activity and transmembrane signaling receptor activity. Acts upstream of or within cellular response to interleukin-4; cellular response to lipopolysaccharide; and cellular response to type II interferon. Located in cell surface. Is expressed in several structures, including hemolymphoid system; intestine; liver; lung; and yolk sac. Orthologous to human MRC1 (mannose receptor C-type 1).

操作步骤


下载本产品说明书

使用方法

  1. 收集细胞,调整细胞浓度到106 - 107个/ml。
  2. 向细胞悬液中加入表染流式抗体,室温避光孵育15分钟。
  3. 加入100 μl FIX & PERM MediumA,震荡混匀,室温避光孵育 15 分钟。
  4. 加入1ml 1× Flow Cytometry Staining Buffer,300 × g离心5分钟,弃上清。
  5. 加入 100 μl FIX & PERM Medium B,再加入5μL Anti-Mouse CD206, APC(Clone: C068C2)流式抗体检测试剂,震荡混匀,室温避光孵育 15 分钟。
  6. 加入 1 ml 1× Flow Cytometry Staining Buffer,300 × g 离心 5 分钟,弃上清。
  7. 加入 500 μl 1× Flow Cytometry Staining Buffer 重悬,上机检测。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

FCM流式检测常见问题

查看更多FCM流式检测相关问题

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