F1101003

Anti-Human CD10, APC (Clone:SN5c) (新品)

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¥792.00¥1,760.00

  • 分子靶点:CD10, MME, NEP
  • 种属:人 (Human)
  • 标记:APC
  • 亚型:Mouse IgG1, κ
  • 克隆号:SN5c

在售SKU, 70-AH01004-20, 70-AH01004-50, 70-AH01004-100, AH01004, 70-F1101003-25, 70-F1101003-100

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描述

商品名

Anti-Human CD10, APC (Clone:SN5c)

靶点

CD10, MME, NEP

标记

APC

仪器

适用633nm激光器的流式细胞仪

亚型

Mouse IgG1, κ

克隆号

SN5c

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD10, APC (Clone:SN5c) - 结果示例图片
结果示例

图 - 人外周血染色 Anti-Human CD10, APC (F1101003)(实线) 和 Mouse IgG1 Isotype Control APC(CMG105) (虚线)的结果。以 FSC/SSC 中的粒细胞设门进行分析。

分子信息

概述人 CD10, MME, NEP 靶点信息

MME 分子靶点信息概述

  • 分子名:MME, membrane metalloendopeptidase
  • 基因家族:CD molecules; M13 metallopeptidases
  • 别名:CALLA; CD10; NEP
  • 全称:neutral endopeptidase; enkephalinase; neprilysin

MME 分子靶点综述

CD10 是 100 kDa 的 II 型跨膜蛋白,CD10 抗原又称普通急性淋巴细胞白血病抗原(CALLA)、中性内肽酶(NEP)、gp100 和脑啡肽酶。正常情况下,它在早期 B 和 T 淋巴样前体、中性粒细胞和各种上皮细胞表面上表达。CD10 在急性淋巴母细胞和滤泡生发中心淋巴瘤等淋巴瘤细胞中也有表达。CD10 是一种内肽酶,参与 B 细胞的发育,也是中性粒细胞炎症反应的介质。

人 Human MME 分子靶点信息

  • 分子名:MME, membrane metalloendopeptidase
  • 别称:
    • atriopeptidase
    • CALLA
    • CD10
    • CMT2T
    • common acute lymphocytic leukemia antigen
    • DKFZp686O16152
    • enkephalinase
    • membrane metallo endopeptidase
    • membrane metallo-endopeptidase
    • membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)
    • membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase)
    • membrane metallo-endopeptidase variant 1
    • membrane metallo-endopeptidase variant 2
    • MGC126681
    • MGC126707
    • NEP
    • neprilysin
    • neprilysin-390
    • neprilysin-411
    • neutral endopeptidase 24.11
    • SCA43
    • SFE
    • skin fibroblast elastase
  • 基因序列:NCBI_Gene: 4311
  • 蛋白序列:UniProtKB: P08473

人 Human MME靶点分子功能(预测)

Enables several functions, including peptidase activity; phospholipid binding activity; and protein homodimerization activity. Involved in several processes, including amyloid-beta clearance by cellular catabolic process; cellular response to UV; and neuropeptide processing. Located in several cellular components, including brush border; early endosome; and trans-Golgi network. Implicated in Alzheimer's disease; Charcot-Marie-Tooth disease axonal type 2T; cerebellar ataxia type 43; cerebral amyloid angiopathy; and membranous glomerulonephritis. Biomarker of cerebral amyloid angiopathy and periodontitis.

操作步骤


下载本产品说明书

使用方法

全血样本:取 100 μl 抗凝血,加入 5 μl Anti-Human CD10, APC 和其他抗体,震荡混匀,于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300× g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号 S1001)重悬,加入 5 μl Anti-Human CD10, APC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

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