F21001d03

Anti-Mouse CD1d, APC(Clone: 20H2) 检测试剂

¥464.00 – ¥1,032.00

说明书

分子靶点：CD1D
种属：小鼠 (Mouse)
标记：APC
亚型：Rat IgG1, κ
克隆号：20H2

在售SKU, F21001d03, 70-F21001d03-25, 70-F21001d03-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
CD1D 分子靶点信息概述
CD1D 分子靶点综述
- 小鼠 Mouse Cd1d1 分子靶点信息
- 小鼠 Mouse Cd1d1 靶点分子功能（预测）

商品名	Anti-Mouse CD1d, APC(Clone: 20H2) 检测试剂
靶点	CD1D
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Rat IgG1, κ
克隆号	20H2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 小鼠脾脏细胞进行染色，左边为Rat IgG2b, APC的结果，右图为Anti-Mouse CD1d, APC的结果。以淋巴细胞设门进行分析。

分子信息

概述小鼠 Cd1d1 靶点信息

CD1D 分子靶点信息概述

分子名：CD1D, CD1d molecule
全称：CD1D antigen, d polypeptide; CD1d antigen

CD1D 分子靶点综述

CD1d是group 2 CD1 molecules的唯一成员。该家族在结构上与主要组织相容性复合体（MHC）蛋白相关，并与β-2-微球蛋白形成异二聚体。CD1蛋白介导自身或微生物来源的主要脂质和糖脂抗原向T细胞的呈递。CD1d基因编码的蛋白质通过细胞质尾部的酪氨酸基序定位于晚期内体和溶酶体。与CD1D相关的疾病包括马尔默分枝杆菌和自身免疫性疾病。

小鼠 Mouse Cd1d1 分子靶点信息

分子名：Cd1d1, CD1d1 antigen
别称：
- AI747460
- CD1.1
- Cd1a
- CD1a antigen
- Cd1d
- CD1d antigen
- expressed sequence AI747460
- Ly-38
- lymphocyte antigen 38
基因序列：NCBI_Gene: 12479
蛋白序列：

小鼠 Mouse Cd1d1 靶点分子功能（预测）

Enables T cell receptor binding activity and endogenous lipid antigen binding activity. Involved in NK T cell differentiation and regulation of immature T cell proliferation in thymus. Acts upstream of or within several processes, including antigen processing and presentation, exogenous lipid antigen via MHC class Ib; positive regulation of cytokine production; and positive regulation of leukocyte activation. Located in endosome; external side of plasma membrane; and lysosome. Is expressed in forebrain; intestine; jaw bone; and liver. Human ortholog(s) of this gene implicated in pulmonary tuberculosis. Orthologous to human CD1D (CD1d molecule).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入5 μlAnti-Human CD1d, APC和其他抗体，使反应终体积为 100 μl 。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。