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KTH001

Human Th1/Th2/Th17 Staining Kit 人Th1/Th2/Th17染色试剂盒

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¥3,200.00 – ¥12,000.00

70-KTH001-25, 70-KTH001-100,th1, th2, th17, 染色, Th, 分型, 细胞分型, 免疫分型, 检测, 分型检测, 试剂盒

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描述

产品简介

本产品用于检测人抗凝血中Th1、Th2和Th17细胞在T淋巴细胞 (或CD4+辅助T细胞)中的比例。通常所说的Th1、Th2和Th17是指在各种生理与病理条件下有能力分化为Th1、Th2和Th17 的T辅助细胞 (严格意义上是Th0细胞)。静息状态 (即未受任何刺激，如人的正常生理状态)下，Th0分化为Th1、Th2和Th17的能力非常弱，所以外周血中仅含有极少量的Th1、Th2和Th17细胞，这时所能检测到的IFN-γ、IL-4和IL-17A也微乎其微。而当Th细胞受到外界因素 (如刺激素、病原体等)刺激，其中Th0即会向Th1、Th2或Th17分化，具体分化趋向取决于细胞因子的种类。此时，检测到的IFN-γ、IL-4或IL-17A也较多。实验中检测的Th1、Th2和Th17实际上是检测Th细胞对刺激素刺激的反应能力。

产品特点

  1. 即用型，无需购买其他产品；
  2. 灵敏度高，特异性强，结果稳定；
  3. 价格便宜，现货供应。

结果示例

Flow cytometric analysis of Human Th1/Th2/Th17 Staining Kit. The staining pattern of IFN-g , IL-4 and IL-17A on resting heparin anticoagulated blood (A0, B0 and C0). PMA/Ionomycin stimulated heparin anticoagulated blood (A1, B1 and C1). Dot plot analysis are derived from gated CD3+/CD8- cells population. Flow cytometry was performed on a Thermo Fisher Attune NxT. The health status of the volunteers is unclear.

使用Human Th1/Th2/Th17 Staining Kit进行流式检测。静息的肝素抗凝血(A0、B0 和C0)染色IFN-g、IL-4和IL-17A。PMA/Ionomycin刺激的肝素抗凝血(A1、B1和C1)染色IFN-g和IL-4。对CD3+/CD8-细胞进行设门分析。实验在Thermo Fisher公司的Attune NxT流式细胞仪上进行。志愿者的健康状况未详。

试剂盒组分

组分 编号 25Test 100Test
抗人CD3, APC-Cy7 (克隆号：UCHT1) AH0030311 150 μl 600 μl
抗人CD8α, PerCP-Cy5.5 (克隆号：RPA-T8) AH008A07 150 μl 600 μl
抗人IFN-γ, FITC (克隆号：4S.B3) AH0IF01 150 μl 600 μl
抗人IL-4, APC (克隆号：8D4-8) AH0I405 150 μl 600 μl
抗人IL-17A, PE (克隆号：64DEC17) AH0I1704 150 μl 600 μl
佛波酯/离子霉素混合物 (250×) CS1001 100 μl 200 μl
布雷非德菌素A/莫能霉素混合物 (250×) CS1002 100 μl 200 μl
固定破膜剂试剂A GASA 3 ml 11 ml
固定破膜剂试剂B GASB 3 ml 11 ml
流式染色缓冲液 (10×) S1002 15 ml 60 ml

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