F1102003

Anti-Human CD20,APC (Clone: 2H7) 流式抗体 (新品)

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¥872.00¥1,936.00

  • 分子靶点:CD20, Bp35, FMC7, MS4A1
  • 种属:人 (Human)
  • 标记:APC
  • 亚型:Mouse IgG2b, κ
  • 克隆号:2H7

在售SKU, 70-AH02005-20, 70-AH02005-50, 70-AH02005-100, AH02005, 70-F1102003-25, 70-F1102003-100

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描述

商品名

Anti-Human CD20,APC (Clone: 2H7) 流式抗体

靶点

CD20, Bp35, FMC7, MS4A1

标记

APC

仪器

适用633nm激光器的流式细胞仪

亚型

Mouse IgG2b, κ

克隆号

2H7

推荐应用

流式检测

用量

5 μL /Test

纯化

亲和层析

储存液

含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)

保存条件

2 - 8℃避光,切勿冻存

结果示例

Anti-Human CD20,APC (Clone: 2H7) 流式抗体 - 结果示例图片
结果示例

图 - 人外周血染色 Anti-Human CD20, APC (F1102003) 和 Anti-Human CD3, FITC (F1100301) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD20, Bp35, FMC7, MS4A1 靶点信息

MS4A1 分子靶点信息概述

  • 分子名:MS4A1, membrane spanning 4-domains A1
  • 基因家族:CD molecules; Membrane spanning 4-domains
  • 别名:B1; Bp35; FMC7
  • 曾用名:CD20
  • 全称:membrane-spanning 4-domains, subfamily A, member 1

MS4A1 分子靶点综述

CD20是一种分子量为33-37 kD的磷酸化蛋白质分子,表达在B淋巴细胞及其前体,但最早期的祖细胞不表达、滤泡树突状细胞及小部分T细胞弱表达。它主要参与调节B淋巴细胞的增殖与分化,在免疫系统起重要作用。由于CD20在恶性B细胞表面的表达特点及与抗体结合不发生内吞作用,已经将其作为免疫治疗霍奇金淋巴瘤的靶点。

人 Human MS4A1 分子靶点信息

  • 分子名:MS4A1, membrane spanning 4-domains A1
  • 别称:
    • B-lymphocyte antigen CD20
    • B-lymphocyte cell-surface antigen B1
    • B-lymphocyte surface antigen B1
    • B1
    • Bp35
    • CD20
    • CD20 antigen
    • CD20 receptor
    • CVID5
    • FMC7
    • LEU-16
    • leukocyte surface antigen Leu-16
    • membrane-spanning 4-domains subfamily A member 1
    • membrane-spanning 4-domains, subfamily A, member 1
    • MGC3969
    • MS4A2
    • S7
  • 基因序列:NCBI_Gene: 931
  • 蛋白序列:UniProtKB: P11836

人 Human MS4A1靶点分子功能(预测)

Enables identical protein binding activity and immunoglobulin binding activity. Involved in several processes, including B cell receptor signaling pathway; calcium ion transport; and protein tetramerization. Located in cell surface; nucleoplasm; and plasma membrane raft. Implicated in common variable immunodeficiency.

操作步骤


下载本产品说明书

使用方法

全血样本:取 100 μl 抗凝血,加入 5 μl Anti-Human CD20, APC 和其他抗体,震荡混匀,于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作,去除红细胞。300× g 离心 5 - 10 分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
其他样本:将 1×105 - 108 个单细胞经适当离心后,取适量流式染色缓冲液 (目录号 S1001)重悬,加入 5 μl Anti-Human CD20, APC 和其他抗体,使反应终体积为 100 μl。震荡混匀,于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液,300 × g 离心 5 - 10分钟,弃上清,加入 500 μl 流式染色缓冲液重悬,上机检测。
实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

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