F1102003

Anti-Human CD20，APC (Clone: 2H7) 流式抗体 (新品)

¥872.00 – ¥1,936.00

说明书

分子靶点：CD20, Bp35, FMC7, MS4A1
种属：人 (Human)
标记：APC
亚型：Mouse IgG2b, κ
克隆号：2H7

在售SKU, 70-AH02005-20, 70-AH02005-50, 70-AH02005-100, AH02005, 70-F1102003-25, 70-F1102003-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
MS4A1 分子靶点信息概述
MS4A1 分子靶点综述
- 人 Human MS4A1 分子靶点信息
- 人 Human MS4A1靶点分子功能（预测）

商品名	Anti-Human CD20，APC (Clone: 2H7) 流式抗体
靶点	CD20, Bp35, FMC7, MS4A1
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Mouse IgG2b, κ
克隆号	2H7
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血染色 Anti-Human CD20, APC (F1102003) 和 Anti-Human CD3, FITC (F1100301) 的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 CD20, Bp35, FMC7, MS4A1 靶点信息

MS4A1 分子靶点信息概述

分子名：MS4A1, membrane spanning 4-domains A1
基因家族：CD molecules; Membrane spanning 4-domains
别名：B1; Bp35; FMC7
曾用名：CD20
全称：membrane-spanning 4-domains, subfamily A, member 1

MS4A1 分子靶点综述

CD20是一种分子量为33-37 kD的磷酸化蛋白质分子，表达在B淋巴细胞及其前体，但最早期的祖细胞不表达、滤泡树突状细胞及小部分T细胞弱表达。它主要参与调节B淋巴细胞的增殖与分化，在免疫系统起重要作用。由于CD20在恶性B细胞表面的表达特点及与抗体结合不发生内吞作用，已经将其作为免疫治疗霍奇金淋巴瘤的靶点。

人 Human MS4A1 分子靶点信息

分子名：MS4A1, membrane spanning 4-domains A1
别称：
- B-lymphocyte antigen CD20
- B-lymphocyte cell-surface antigen B1
- B-lymphocyte surface antigen B1
- B1
- Bp35
- CD20
- CD20 antigen
- CD20 receptor
- CVID5
- FMC7
- LEU-16
- leukocyte surface antigen Leu-16
- membrane-spanning 4-domains subfamily A member 1
- membrane-spanning 4-domains, subfamily A, member 1
- MGC3969
- MS4A2
- S7
基因序列：NCBI_Gene: 931
蛋白序列：UniProtKB: P11836

人 Human MS4A1靶点分子功能（预测）

Enables identical protein binding activity and immunoglobulin binding activity. Involved in several processes, including B cell receptor signaling pathway; calcium ion transport; and protein tetramerization. Located in cell surface; nucleoplasm; and plasma membrane raft. Implicated in common variable immunodeficiency.

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD20, APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300× g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD20, APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。