EK282HS

Mouse TNF-α High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：TNF, TNFA, TNFSF2
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：0.32 pg/mL
检测范围：0.781 pg/mL - 50 pg/mL
回收率：75% - 113%

在售SKU:70-EK282HS-48, 70-EK282HS-96, EK282HS

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库存号

描述

文章目录[隐藏]

分子信息
TNF 分子靶点信息概述
TNF 分子靶点综述
- 小鼠 Mouse Tnf 分子靶点信息
- 小鼠 Mouse Tnf 靶点分子功能（预测）

商品名	Mouse TNF-α High Sensitivity ELISA Kit (小鼠TNF-α高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.6% - 4.9%；板间变异系数：5.7% - 8.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	0.32 pg/mL
检测范围	0.781 pg/mL - 50 pg/mL
回收率	75% - 113%
平均回收率	0.91
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TNF-α单克隆抗体的96孔聚苯乙烯酶标板小鼠TNF-α冻干标准品 TNF-α检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的TNF-α与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse TNF-α High Sensitivity ELISA Kit (小鼠TNF-α高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

分子名：TNF, tumor necrosis factor
基因家族：Tumor necrosis factor superfamily
别名：TNFSF2; DIF; TNF-alpha
曾用名：TNFA
全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

小鼠 Mouse Tnf 分子靶点信息

分子名：Tnf, tumor necrosis factor
别称：
- DIF
- TNF alpha
- TNF-alpha
- Tnfa
- TNFalpha
- Tnfsf1a
- tumor necrosis factor-alpha
- tumor necrosis factor, alpha
基因序列：NCBI_Gene: 21926
蛋白序列：

小鼠 Mouse Tnf 靶点分子功能（预测）

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).