F11ZAP01

Anti-Human ZAP-70, FITC (Clone:1E7.2)检测试剂

¥784.00 – ¥1,728.00

说明书

分子靶点：ZAP70, STD, SRK
种属：人 (Human)
标记：FITC
亚型：Mouse IgG1, κ
克隆号：1E7.2

在售SKU, 70-AH0Z01-20, 70-AH0Z01-50, 70-AH0Z01-100, AH0Z01, 70-F11ZAP01-25, 70-F11ZAP01-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
ZAP70 分子靶点信息概述
ZAP70 分子靶点综述
- 人 Human ZAP70 分子靶点信息
- 人 Human ZAP70靶点分子功能（预测）

商品名	Anti-Human ZAP-70, FITC (Clone:1E7.2)检测试剂
靶点	ZAP70, STD, SRK
标记	FITC
仪器	适用488nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	1E7.2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 人外周血经 Anti-Human CD3, PE (F11003A02) 染色后，固定破膜染色Anti-Human ZAP-70, FITC(F11ZAP01)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 ZAP70, STD, SRK 靶点信息

ZAP70 分子靶点信息概述

分子名：ZAP70, zeta chain of T cell receptor associated protein kinase 70
基因家族：SH2 domain containing; Syk family tyrosine kinases
别名：ZAP-70; STD
曾用名：SRK
全称：tyrosine-protein kinase ZAP-70; SRK

ZAP70 分子靶点综述

TCR 相关蛋白-70，在 T 细胞和 NK 细胞中表达，是 TCR信号传递和发育所必需的。ZAP-70 通过与 TCR 复合物不变亚基中存在的 ITAMs 结合，与 TCR 复合物相互作用。激活后，ZAP-70通过两种机制在几个酪氨酸残基上磷酸化：自磷酸化和 Src 家族酪氨酸激酶Lck1-3的反式磷酸化。酪氨酸磷酸化 ZAP-70 与其增加激酶活性有关，并触发下游信号事件。虽然 ZAP-70 通常在T 细胞和 NK 细胞中表达，但最近的几项研究也显示 ZAP-70 阳性表达与突变 IgVH 在 B 慢性淋巴细胞白血病(CCL)中的表达高度相关。总之，ZAP-70 的表达可以通过细胞内流式细胞术来测量，可以作为 B-CLL 的预后指标。

人 Human ZAP70 分子靶点信息

分子名：ZAP70, zeta chain of T cell receptor associated protein kinase 70
别称：
- 70 kDa zeta-associated protein
- 70 kDa zeta-chain associated protein
- ADMIO2
- FLJ17670
- FLJ17679
- IMD48
- SRK
- STCD
- STD
- syk-related tyrosine kinase
- tyrosine-protein kinase ZAP-70
- TZK
- ZAP-70
- zeta chain of T cell receptor associated protein kinase 70kDa
- zeta chain of T-cell receptor associated protein kinase 70
- zeta-chain (TCR) associated protein kinase 70kDa
- zeta-chain associated protein kinase, 70kD
基因序列：NCBI_Gene: 7535
蛋白序列：UniProtKB: P43403

人 Human ZAP70靶点分子功能（预测）

Enables non-membrane spanning protein tyrosine kinase activity and phosphotyrosine residue binding activity. Involved in immune response and protein phosphorylation. Acts upstream of or within peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; and positive thymic T cell selection. Located in plasma membrane. Part of T cell receptor complex. Colocalizes with membrane raft. Implicated in immunodeficiency 48 and severe combined immunodeficiency.

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

取 100 μl 抗凝血或 1×10⁵ - 10⁸ 个单细胞悬液，按照相应抗体说明书进行表面染色、离心洗涤。
每管加入 100 μl FIX&PERM Kit A 液，涡旋振荡混匀，室温避光孵育 15 min。
孵育结束后，每管加入 2 ml 1×Flow Cytometry Staining Buffer，涡旋振荡混匀，1500 rpm 离心 5 min，弃上清。
每管加入 100 μl FIX&PERM Kit B 液和 5 μl Anti-Human ZAP-70, FITC 抗体涡旋振荡混匀，室温避光孵育 15 min。
孵育结束后，每管加入 2 ml 1×Flow Cytometry Staining Buffer，涡旋振荡混匀，1500 rpm 离心 5 min，弃上清。
每管加入 500 μl 1× Flow Cytometry Staining Buffer 重悬，上机检测。

实验中，可对抗体进行优化滴定以获得最佳效果。