F11ZAP01

Anti-Human ZAP-70, FITC (Clone:1E7.2)检测试剂

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  • 分子靶点:ZAP70, STD, SRK
  • 种属:人 (Human)
  • 标记:FITC
  • 亚型:Mouse IgG1, κ
  • 克隆号:1E7.2

在售SKU, 70-AH0Z01-20, 70-AH0Z01-50, 70-AH0Z01-100, AH0Z01, 70-F11ZAP01-25, 70-F11ZAP01-100

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描述

商品名 Anti-Human ZAP-70, FITC (Clone:1E7.2)检测试剂
靶点 ZAP70, STD, SRK
标记 FITC
仪器 适用488nm激光器的流式细胞仪
亚型 Mouse IgG1, κ
克隆号 1E7.2
推荐应用 流式检测
用量 5 μL /Test
纯化 亲和层析
储存液 含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件 2 - 8℃避光,切勿冻存

结果示例

Anti-Human ZAP-70, FITC (Clone:1E7.2) - 结果示例图片
结果示例

图 - 人外周血经 Anti-Human CD3, PE (F11003A02) 染色后,固定破膜染色Anti-Human ZAP-70, FITC(F11ZAP01)的结果。以 FSC/SSC 中的淋巴细胞设门进行分析。

分子信息

概述人 ZAP70, STD, SRK 靶点信息

ZAP70 分子靶点信息概述

  • 分子名:ZAP70, zeta chain of T cell receptor associated protein kinase 70
  • 基因家族:SH2 domain containing; Syk family tyrosine kinases
  • 别名:ZAP-70; STD
  • 曾用名:SRK
  • 全称:tyrosine-protein kinase ZAP-70; SRK

ZAP70 分子靶点综述

TCR 相关蛋白-70,在 T 细胞和 NK 细胞中表达,是 TCR信号传递和发育所必需的。ZAP-70 通过与 TCR 复合物不变亚基中存在的 ITAMs 结合,与 TCR 复合物相互作用。激活后,ZAP-70通过两种机制在几个酪氨酸残基上磷酸化:自磷酸化和 Src 家族酪氨酸激酶Lck1-3的反式磷酸化。酪氨酸磷酸化 ZAP-70 与其增加激酶活性有关,并触发下游信号事件。虽然 ZAP-70 通常在T 细胞和 NK 细胞中表达,但最近的几项研究也显示 ZAP-70 阳性表达与突变 IgVH 在 B 慢性淋巴细胞白血病(CCL)中的表达高度相关。总之,ZAP-70 的表达可以通过细胞内流式细胞术来测量,可以作为 B-CLL 的预后指标。

人 Human ZAP70 分子靶点信息

  • 分子名:ZAP70, zeta chain of T cell receptor associated protein kinase 70
  • 别称:
    • 70 kDa zeta-associated protein
    • 70 kDa zeta-chain associated protein
    • ADMIO2
    • FLJ17670
    • FLJ17679
    • IMD48
    • SRK
    • STCD
    • STD
    • syk-related tyrosine kinase
    • tyrosine-protein kinase ZAP-70
    • TZK
    • ZAP-70
    • zeta chain of T cell receptor associated protein kinase 70kDa
    • zeta chain of T-cell receptor associated protein kinase 70
    • zeta-chain (TCR) associated protein kinase 70kDa
    • zeta-chain associated protein kinase, 70kD
  • 基因序列:NCBI_Gene: 7535
  • 蛋白序列:UniProtKB: P43403

人 Human ZAP70靶点分子功能(预测)

Enables non-membrane spanning protein tyrosine kinase activity and phosphotyrosine residue binding activity. Involved in immune response and protein phosphorylation. Acts upstream of or within peptidyl-tyrosine phosphorylation; positive regulation of T cell differentiation; and positive thymic T cell selection. Located in plasma membrane. Part of T cell receptor complex. Colocalizes with membrane raft. Implicated in immunodeficiency 48 and severe combined immunodeficiency.

操作步骤


下载本产品说明书

使用方法

  1. 取 100 μl 抗凝血或 1×105 - 108 个单细胞悬液,按照相应抗体说明书进行表面染色、离心洗涤。
  2. 每管加入 100 μl FIX&PERM Kit A 液,涡旋振荡混匀,室温避光孵育 15 min。
  3. 孵育结束后,每管加入 2 ml 1×Flow Cytometry Staining Buffer,涡旋振荡混匀,1500 rpm 离心 5 min,弃上清。
  4. 每管加入 100 μl FIX&PERM Kit B 液和 5 μl Anti-Human ZAP-70, FITC 抗体涡旋振荡混匀,室温避光孵育 15 min。
  5. 孵育结束后,每管加入 2 ml 1×Flow Cytometry Staining Buffer,涡旋振荡混匀,1500 rpm 离心 5 min,弃上清。
  6. 每管加入 500 μl 1× Flow Cytometry Staining Buffer 重悬,上机检测。

实验中,可对抗体进行优化滴定以获得最佳效果。

注意事项

  1. 请在使用本产品前仔细阅读说明书。本产品仅用于科研,不可用于诊断。
  2. 为了您的安全和健康,请穿戴实验防护服、手套、口罩等必要的防护装备。
  3. 更多相关产品敬请关注联科生物网站或来电咨询。

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