EK182S

Human TNF-α Standard (人肿瘤坏死因子α (TNF-α) 标准品)

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¥280.00

  • 分子靶点:TNF, TNFA, TNFSF2
  • 种属:人 (Human)
  • 试剂盒:EK182
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK182S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Human TNF-α Standard (人肿瘤坏死因子α (TNF-α) 标准品)

组分

人TNF-α 标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human TNF-α ELISA Kit (人肿瘤坏死因子α (TNF-α) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名:TNF, tumor necrosis factor
  • 基因家族:Tumor necrosis factor superfamily
  • 别名:TNFSF2; DIF; TNF-alpha
  • 曾用名:TNFA
  • 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

人 Human TNF 分子靶点信息

  • 分子名:TNF, tumor necrosis factor
  • 别称:
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • 基因序列:NCBI_Gene: 7124
  • 蛋白序列:UniProtKB: P01375

人 Human TNF靶点分子功能(预测)

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

操作步骤


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引用文献

文章目录[隐藏]


  1. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

  2. RAW 264.7 人肺癌细胞 A549 支气管肺泡灌洗液 BALF 细胞培养上清

    Melatonin attenuates LPS-induced pyroptosis in acute lung injury by inhibiting NLRP3-GSDMD pathway via activating Nrf2/HO-1 signaling axis 

  3. 细胞培养上清 脐带静脉内皮细胞 血清

    Cd137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating Nf-Κb Pathway 

  4. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  5. 细胞培养上清

    Toxoplasma Gondii Sag1 Targeting Host Cell S100a6 for Parasite Invasion and Host Immunity 

  6. 细胞培养上清

    Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 

  7. 血清

    Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

  8. T细胞 细胞培养上清 肿瘤

    Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer 

  9. 细胞培养上清

    Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I 

  10. Hjurp Promotes Hepatocellular Carcinoma Proliferation by Destabilizing P21 Via the Mapk/Erk1/2 and Akt/Gsk3β Signaling Pathways 

  11. RAW 264.7

    Influences of Surface Coating of Plga Nanoparticles on Immune Activation of Macrophages 

  12. Involvement of Polyphosphate Kinase in Virulence and Stress Tolerance of Uropathogenic Proteus Mirabilis 

  13. A Fusion Receptor as a Safety Switch, Detection, and Purification Biomarker for Adoptive Transferred T Cells 

  14. Anti-B7-H3 Monoclonal Antibody Ameliorates the Damage of Acute Experimental Pancreatitis by Attenuating the Inflammatory Response 

  15. Interleukin 1β and Tumor Necrosis Factor Α Promote Hfob1.19 Cell Viability Via Activating Ap1 

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