Human TNF-α Standard (人肿瘤坏死因子α (TNF-α) 标准品)
¥280.00
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK182
- ELISA试剂盒
Human TNF-a ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥10,800.00
商品名 |
Human TNF-α Standard (人肿瘤坏死因子α (TNF-α) 标准品) |
---|---|
组分 |
人TNF-α 标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
TNF 分子靶点信息概述
- 分子名:TNF, tumor necrosis factor
- 基因家族:Tumor necrosis factor superfamily
- 别名:TNFSF2; DIF; TNF-alpha
- 曾用名:TNFA
- 全称:TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)
TNF 分子靶点综述
TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能,还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性,调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。
人 Human TNF 分子靶点信息
- 分子名:TNF, tumor necrosis factor
- 别称:
- APC1 protein
- cachectin
- DIF
- TNF superfamily, member 2
- TNF-a
- TNF-alpha
- TNF, macrophage-derived
- TNF, monocyte-derived
- TNFA
- TNFSF2
- TNLG1F
- tumor necrosis factor alpha
- tumor necrosis factor ligand 1F
- tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor-alpha
- 基因序列:NCBI_Gene: 7124
- 蛋白序列:UniProtKB: P01375
人 Human TNF靶点分子功能(预测)
Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).
操作步骤
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引用文献
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- A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 
- Melatonin attenuates LPS-induced pyroptosis in acute lung injury by inhibiting NLRP3-GSDMD pathway via activating Nrf2/HO-1 signaling axis 
- Cd137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating Nf-Κb Pathway 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Toxoplasma Gondii Sag1 Targeting Host Cell S100a6 for Parasite Invasion and Host Immunity 
- Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 
- Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 
- Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer 
- Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I 
- Hjurp Promotes Hepatocellular Carcinoma Proliferation by Destabilizing P21 Via the Mapk/Erk1/2 and Akt/Gsk3β Signaling Pathways 
- Influences of Surface Coating of Plga Nanoparticles on Immune Activation of Macrophages 
- Involvement of Polyphosphate Kinase in Virulence and Stress Tolerance of Uropathogenic Proteus Mirabilis 
- A Fusion Receptor as a Safety Switch, Detection, and Purification Biomarker for Adoptive Transferred T Cells 
- Anti-B7-H3 Monoclonal Antibody Ameliorates the Damage of Acute Experimental Pancreatitis by Attenuating the Inflammatory Response 
- Interleukin 1β and Tumor Necrosis Factor Α Promote Hfob1.19 Cell Viability Via Activating Ap1 
A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 
- RAW 264.7 人肺癌细胞 A549 支气管肺泡灌洗液 BALF 细胞培养上清
Melatonin attenuates LPS-induced pyroptosis in acute lung injury by inhibiting NLRP3-GSDMD pathway via activating Nrf2/HO-1 signaling axis 
- 细胞培养上清 脐带静脉内皮细胞 血清
Cd137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating Nf-Κb Pathway 
- 腹腔上清液
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- 细胞培养上清
Toxoplasma Gondii Sag1 Targeting Host Cell S100a6 for Parasite Invasion and Host Immunity 
- 细胞培养上清
Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy 
- 血清
Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 
- T细胞 细胞培养上清 肿瘤
Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer 
- 细胞培养上清
Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I 
Hjurp Promotes Hepatocellular Carcinoma Proliferation by Destabilizing P21 Via the Mapk/Erk1/2 and Akt/Gsk3β Signaling Pathways 
- RAW 264.7
Influences of Surface Coating of Plga Nanoparticles on Immune Activation of Macrophages 
Involvement of Polyphosphate Kinase in Virulence and Stress Tolerance of Uropathogenic Proteus Mirabilis 
A Fusion Receptor as a Safety Switch, Detection, and Purification Biomarker for Adoptive Transferred T Cells 
Anti-B7-H3 Monoclonal Antibody Ameliorates the Damage of Acute Experimental Pancreatitis by Attenuating the Inflammatory Response 
Interleukin 1β and Tumor Necrosis Factor Α Promote Hfob1.19 Cell Viability Via Activating Ap1 
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