EK310HS

Rat IL-10 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：IL10, CSIF, TGIF
种属：大鼠 (Rat)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：10 μL
灵敏度：0.22 pg/mL
检测范围：6.25 pg/mL - 400 pg/mL
回收率：94% - 115%

在售SKU:70-EK310HS-48, 70-EK310HS-96

¥<\/span>2,000.00<\/bdi><\/span><\/span>","sku":"70-EK310HS-48","variation_description":"","variation_id":7927,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"},{"attributes":{"attribute_size":"96T"},"availability_html":"","backorders_allowed":false,"dimensions":{"length":"","width":"","height":""},"dimensions_html":"N\/A","display_price":3400,"display_regular_price":3400,"image":{"title":"ELISA-1.jpg","caption":"","url":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","alt":"","src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","srcset":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg 800w, https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-768x576.jpg 768w","sizes":"(max-width: 800px) 100vw, 800px","full_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","full_src_w":800,"full_src_h":600,"gallery_thumbnail_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1-100x100.jpg","gallery_thumbnail_src_w":100,"gallery_thumbnail_src_h":100,"thumb_src":"https:\/\/www.liankebio.com\/wp-content\/uploads\/ELISA-1.jpg","thumb_src_w":800,"thumb_src_h":600,"src_w":800,"src_h":600},"image_id":5986,"is_downloadable":false,"is_in_stock":true,"is_purchasable":true,"is_sold_individually":"no","is_virtual":false,"max_qty":"","min_qty":1,"price_html":"¥<\/span>3,400.00<\/bdi><\/span><\/span>","sku":"70-EK310HS-96","variation_description":"","variation_id":7928,"variation_is_active":true,"variation_is_visible":true,"weight":"","weight_html":"N\/A"}]">

Size
库存号

描述

文章目录[隐藏]

分子信息
IL10 分子靶点信息概述
IL10 分子靶点综述
- 大鼠 Rat Il10 分子靶点信息
- 大鼠 Rat Il10 靶点分子功能（预测）

商品名	Rat IL-10 High Sensitivity ELISA Kit (大鼠白细胞介素10 高敏 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.4% - 4.8%；板间变异系数：3.9% - 4.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	10 μL
灵敏度	0.22 pg/mL
检测范围	6.25 pg/mL - 400 pg/mL
回收率	94% - 115%
平均回收率	1.03
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板大鼠IL-10冻干标准品 IL-10检测抗体标准品稀释液辣根过氧化物酶标记的链霉亲和素信号增强剂浓缩液信号增强剂稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-10与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-10 High Sensitivity ELISA Kit (大鼠白细胞介素10 高敏 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述大鼠 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

分子名：IL10, interleukin 10
基因家族：Interleukins
别名：CSIF; TGIF; IL10A; IL-10
全称：cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL-10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

大鼠 Rat Il10 分子靶点信息

分子名：Il10, interleukin 10
别称：
- CSIF
- cytokine synthesis inhibitory factor
- If2a
- IL-10
- IL10X
- interferon 2a
- interleukin-10
基因序列：NCBI_Gene: 25325
蛋白序列：
- UniprotKB: A0A7R8C3K4
- UniprotKB: P29456

大鼠 Rat Il10 靶点分子功能（预测）

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).