EK310HS

Rat IL-10 High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

  • 分子靶点:IL10, CSIF, TGIF
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:10 μL
  • 灵敏度:0.22 pg/mL
  • 检测范围:6.25 pg/mL - 400 pg/mL
  • 回收率:94% - 115%

在售SKU:70-EK310HS-48, 70-EK310HS-96

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描述

商品名

Rat IL-10 High Sensitivity ELISA Kit (大鼠白细胞介素10 高敏 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.4% - 4.8%;板间变异系数:3.9% - 4.9%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

10 μL

灵敏度

0.22 pg/mL

检测范围

6.25 pg/mL - 400 pg/mL

回收率

94% - 115%

平均回收率

1.03

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠IL-10冻干标准品
  • IL-10检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-10与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat IL-10 High Sensitivity ELISA Kit (大鼠白细胞介素10 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 IL10, CSIF, TGIF 靶点信息

IL10 分子靶点信息概述

  • 分子名:IL10, interleukin 10
  • 基因家族:Interleukins
  • 别名:CSIF; TGIF; IL10A; IL-10
  • 全称:cytokine synthesis inhibitory factor; T-cell growth inhibitory factor

IL10 分子靶点综述

白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中,它由位于1号染色体的IL-10基因编码,由5个外显子组成。IL-10主要由单核细胞分泌,淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性,并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床,抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。

大鼠 Rat Il10 分子靶点信息

大鼠 Rat Il10 靶点分子功能(预测)

Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).

操作步骤


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