EK176

Human IgM ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

  • 分子靶点:待定
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞上清
  • 检测样本体积:100 μL
  • 灵敏度:1.37 ng/mL
  • 检测范围:15.63 ng/mL - 1000 ng/mL
  • 回收率:92% - 110%

在售SKU:70-EK176-48, 70-EK176-96

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描述

商品名

Human IgM ELISA Kit (人免疫球蛋白M ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 7.7%;板间变异系数:5.0% - 10.1%

样本类型

血清,血浆,细胞上清

检测样本体积

100 μL

灵敏度

1.37 ng/mL

检测范围

15.63 ng/mL - 1000 ng/mL

回收率

92% - 110%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IgM单克隆抗体的96孔聚苯乙烯酶标板
  • 人IgM冻干标准品
  • IgM检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IgM抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本进行孵育。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的检测抗体孵育。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IgM的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IgM ELISA Kit (人免疫球蛋白M ELISA试剂盒) - 标准曲线
标准曲线

操作步骤


下载本产品说明书

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引用文献


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  2. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

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